DDAH2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-864

Novus Biologicals, part of Bio-Techne
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NB100-864
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all DDAH2 Antibodies »

Product Specifications

Immunogen

Peptide with sequence SSLCLVLSTRPHS corresponding to C-Terminus according to NP_039268.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for DDAH2 Antibody

Western Blot: DDAH2 Antibody [NB100-864]

Western Blot: DDAH2 Antibody [NB100-864]

Western Blot: DDAH2 Antibody [NB100-864] - Staining of Human Lung lysate with antibody at 0.1 ug/mL (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: DDAH2 Antibody [NB100-864]

Immunocytochemistry/ Immunofluorescence: DDAH2 Antibody [NB100-864]

Immunocytochemistry/Immunofluorescence: DDAH2 Antibody [NB100-864] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry: DDAH2 Antibody [NB100-864]

Flow Cytometry: DDAH2 Antibody [NB100-864]

Flow Cytometry: DDAH2 Antibody [NB100-864] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for DDAH2 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:16000

Western Blot

0.1 - 0.3 ug/mL
Application Notes
WB: Approx. 28 kDa band observed in human lung lysates (calculated MW of 29.6 kDa band according to NP_039268.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DDAH2

DDAH2 plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. Overexpression of DDAH2 has also been linked to increased VEGF mRNA expression and enhanced tube formation by cells grown in a 3-dimensional medium.

Alternate Names

DDAHII, dimethylargininase-2, dimethylarginine dimethylaminohydrolase 2G6a, dimethylarginine dimethylaminohydrolase II, EC 3.5.3.18, G6a, N(G), N(G)-dimethylarginine dimethylaminohydrolase 2, NG30, NG-dimethylarginine dimethylamino hydrolase homolog, S-phase protein

Entrez Gene IDs

23564 (Human); 51793 (Mouse)

Gene Symbol

DDAH2

UniProt

NP_039268. (Human)

Additional DDAH2 Products

Product Documents for DDAH2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for DDAH2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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