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DDHD2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82965

Novus Biologicals, part of Bio-Techne
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NBP1-82965
NBP1-82965-25ul

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RMHLELREGLTRMSMDLKNNLLGSLRMAWKSFTRAPYPALQASETPEETEAEPESTSEKPSDVNTEETSVAVKEEVLPINVGMLNGGQRIDYVLQEK

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (85%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DDHD2 Antibody

Western Blot: DDHD2 Antibody [NBP1-82965]

Western Blot: DDHD2 Antibody [NBP1-82965]

Western Blot: DDHD2 Antibody [NBP1-82965] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/ Immunofluorescence: DDHD2 Antibody [NBP1-82965]

Immunocytochemistry/ Immunofluorescence: DDHD2 Antibody [NBP1-82965]

Immunocytochemistry/Immunofluorescence: DDHD2 Antibody [NBP1-82965] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunohistochemistry-Paraffin: DDHD2 Antibody [NBP1-82965]

Immunohistochemistry-Paraffin: DDHD2 Antibody [NBP1-82965]

Immunohistochemistry-Paraffin: DDHD2 Antibody [NBP1-82965] - Staining of human small intestine shows strong positivity in glandular cells.

Applications for DDHD2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DDHD2

Phospholipase that hydrolyzes preferentially phosphatidic acid and phosphatidylethanolamine. May be involved in the maintenance of the endoplasmic reticulum and/or Golgi structures

Alternate Names

DDHD domain containing 2, DDHD domain-containing protein 2, EC 3.1.1.-, KIAA0725PA-PLA1 like, phospholipase DDHD2, SAM, WWE and DDHD domain containing 1, SAM, WWE and DDHD domain-containing protein 1, SAMWD1, sec23p-interacting protein p125-like phosphatidic acid-preferring phospholipaseA1

Gene Symbol

DDHD2

Additional DDHD2 Products

Product Documents for DDHD2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DDHD2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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