DDO Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85884

Novus Biologicals, part of Bio-Techne
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NBP1-85884
NBP1-85884-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DDO Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KGDWNLSPDAENSREILSRCCALEPSLHGACNIREKVGLRPYRPGVRLQTELLARDGQRLPVVHHYGHGSGGISVHWGTALEAARLVSECVHALR

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (82%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DDO Antibody

Western Blot: DDO Antibody [NBP1-85884]

Western Blot: DDO Antibody [NBP1-85884]

Western Blot: DDO Antibody [NBP1-85884] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-150
Immunocytochemistry/ Immunofluorescence: DDO Antibody [NBP1-85884]

Immunocytochemistry/ Immunofluorescence: DDO Antibody [NBP1-85884]

Immunocytochemistry/Immunofluorescence: DDO Antibody [NBP1-85884] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Immunohistochemistry-Paraffin: DDO Antibody [NBP1-85884]

Immunohistochemistry-Paraffin: DDO Antibody [NBP1-85884]

Immunohistochemistry-Paraffin: DDO Antibody [NBP1-85884] - Staining of human cerebral cortex.
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Applications for DDO Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DDO

OXDD (D-aspartate oxidase) is a peroxisomal flavoprotein that selectively catalyzes the oxidative deamination of D-aspartate and its N-methylated derivative, N-methyl D-aspartate. Flavin adenine dinucleotide or 6-hydroxyflavin adenine dinucleotide can serve as the cofactor in this reaction. Two transcript variants encoding different isoforms have been found.

Alternate Names

aspartic oxidase, DASOX, D-aspartate oxidase, DDO-1, DDO-2, EC 1.4.3.1, FLJ45203

Gene Symbol

DDO

Additional DDO Products

Product Documents for DDO Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DDO Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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