DDX1 Antibody (3E5E2) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61745

Novus Biologicals, part of Bio-Techne
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NBP2-61745
NBP2-61745-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3E5E2

Format

BSA Free

Concentration

1 mg/ml

View all DDX1 Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human DDX1 (AA: 642-740) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

82.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DDX1 Antibody (3E5E2) - BSA Free

Western Blot: DDX1 Antibody (3E5E2)BSA Free [NBP2-61745]

Western Blot: DDX1 Antibody (3E5E2)BSA Free [NBP2-61745]

Western Blot: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis using DDX1 mAb against HEK293 (1) and DDX1 (AA: 642-740)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: DDX1 Antibody (3E5E2) - BSA Free [NBP2-61745]

Immunocytochemistry/ Immunofluorescence: DDX1 Antibody (3E5E2) - BSA Free [NBP2-61745]

Immunocytochemistry/Immunofluorescence: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis of Hela cells using DDX1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Immunohistochemistry-Paraffin: DDX1 Antibody (3E5E2) - BSA Free [NBP2-61745]

Immunohistochemistry-Paraffin: DDX1 Antibody (3E5E2) - BSA Free [NBP2-61745]

Immunohistochemistry-Paraffin: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis of bladder cancer tissues using DDX1 mouse mAb with DAB staining.
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Applications for DDX1 Antibody (3E5E2) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
Use in Immunoprecipitation reported in scientific literature (PMID:32755435)

Reviewed Applications

Read 1 review rated 5 using NBP2-61745 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DDX1

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein of unknown function. It shows high transcription levels in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin.;

Long Name

DEAD-Box Helicase 1

Alternate Names

DBP-RB, DEAD Box-1, DEAD/H-Box Helicase 1, UKVH5d

Gene Symbol

DDX1

Additional DDX1 Products

Product Documents for DDX1 Antibody (3E5E2) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DDX1 Antibody (3E5E2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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