DDX1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB200-348

Novus Biologicals, part of Bio-Techne
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NB200-348
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation (Negative), Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all DDX1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 500 and 550 of human DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 1 using the numbering given in entry AAH12739.1 (GeneID 1653).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DDX1 Antibody

Western Blot: DDX1 Antibody [NB200-348]

Western Blot: DDX1 Antibody [NB200-348]

Western Blot: DDX1 Antibody [NB200-348] - Whole cell lysate (20, 40 and 80 ug) from HeLa cells. Antibody used at 0.03 ug/ml.
Immunohistochemistry: DDX1 Antibody [NB200-348]

Immunohistochemistry: DDX1 Antibody [NB200-348]

Immunohistochemistry: DDX1 Antibody [NB200-348] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DDX1 used at a dilution of 1:200 ( 1ug/ml). Detection: DAB

Applications for DDX1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:1000-1:10000
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows. Western Blot: 1:1,000 to 1:10,000. In some cases, the antibody may be diluted further than indicated.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DDX1

DEAD box proteins, a family of putative RNA helicases, are characterized by eight conserved amino acid motifs that are arranged in a core region as found in the prototypical member of the family, eIF-4A. The family's name is derived from the amino acid sequence Asp-Glu-Ala-Asp (DEAD) that is located within the ATP hydrolysis motif. DEAD box proteins have been implicated in translation initiation and RNA splicing, degradation, and stability. The DEAD box protein, DDX1, was identified in retinoblastoma cell lines, where it is coamplified with MYCN, a member of the MYC transcription factor family. DDX1 is expressed at elevated levels in fetal tissues of neural origin (retina and brain). It is both cytoplasmic and nuclear in DDX1-amplified neuroblastoma and retinoblastoma cell lines. However, it localizes specifically to the nucleus in non-amplified cell lines. Thus, DDX1 is thought to be involved in nuclear RNA metabolism. In addition, the decrease in mean disease-free survival of neuroblastoma patients exhibiting coamplification of DDX1 and MYCN as compared to those exhibiting MYCN amplification indicates a possible role for DDX1 in cancer formation in neural tissues.

Long Name

DEAD-Box Helicase 1

Alternate Names

DBP-RB, DEAD Box-1, DEAD/H-Box Helicase 1, UKVH5d

Entrez Gene IDs

1653 (Human)

Gene Symbol

DDX1

Additional DDX1 Products

Product Documents for DDX1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DDX1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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