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DDX18 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-58939

Novus Biologicals, part of Bio-Techne
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NBP2-58939

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: ETMGSRKVKKSKQKPMNVGLSETQNGGMSQEAVGNIKVTKSPQKSTVLTNGEAAMQSSNSES

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DDX18 Antibody

Western Blot: DDX18 Antibody [NBP2-58939]

Western Blot: DDX18 Antibody [NBP2-58939]

Western Blot: DDX18 Antibody [NBP2-58939] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.
Immunocytochemistry/ Immunofluorescence: DDX18 Antibody [NBP2-58939]

Immunocytochemistry/ Immunofluorescence: DDX18 Antibody [NBP2-58939]

Immunocytochemistry/Immunofluorescence: DDX18 Antibody [NBP2-58939] - Staining of human cell line A-431 shows localization to nucleoli.

Applications for DDX18 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DDX18

DDX18, also known as ATP-dependent RNA helicase DDX18, is a 75.4 kDa 670 amino acid protein which is involved in unwinding RNA as a part of the DEAD box protein family. Current research is linking the protein to diseases and disorders such as malaria, arthritis, lung cancer, and neuroblastoma. The protein is involved in transcriptional activation pathways where it interacts with proteins such as HIST1H4A and HIST1H4B.

Alternate Names

DEAD (Asp-Glu-Ala-Asp) box polypeptide 18, DEAD box protein 18, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 18 (Myc-regulated), EC 3.6.1, EC 3.6.4.13, FLJ33908, MrDbATP-dependent RNA helicase DDX18, Myc-regulated DEAD box protein

Gene Symbol

DDX18

Additional DDX18 Products

Product Documents for DDX18 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DDX18 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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