DDX19B Antibody

Western Blot: DDX19B Antibody [NB100-760]

Western Blot: DDX19B Antibody [NB100-760] - Ddx19 is specifically required for the nuclear localization of MKL1. Western blotting (WB) of cells treated with control (Ctrl) or Ddx19 siRNAs (si). Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms6978), licensed under a CC-BY license.

Western Blot: DDX19B Antibody [NB100-760]

Western Blot: DDX19B Antibody [NB100-760] - Ddx19 interacts with MKL1 RPEL domain and Ipobeta. Ddx19 recruitment from Hela cell lysate by GST-RPEL and GST-Ipobeta (left). WB, western blotting; (-), without Ran-Q69L; (+), with RanQ69L. Corresponding GST baits are stained with Ponceau to ensure the equal loading of the samples (below). Input sample corresponds to 5% of the Hela cell lysate used in the assay. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms6978), licensed under a CC-BY license.

Western Blot: DDX19B Antibody [NB100-760]

Western Blot: DDX19B Antibody [NB100-760] - Ddx19 interacts with MKL1 RPEL domain and Ipobeta. GST-RPEL was used as a bait for pull down of Ddx19 and Ipobeta from the Hela cell lysate in the presence of increasing amounts of LatB-actin (0.25-10 uM) (left). Arrow indicates the increasing amounts of actin in the corresponding Ponceau staining (below). Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms6978), licensed under a CC-BY license.

Western Blot: DDX19B Antibody [NB100-760]

Western Blot: DDX19B Antibody [NB100-760] - MKL1 contains two Ddx19 interaction sites. HA-tagged MKL1 constructs were immunoprecipitated from NIH 3T3 lysates and their expression was detected by HA-antibody. Co-precipitated endogenous Ddx19 was detected by western blotting (WB). Empty HA was used as a negative control. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms6978), licensed under a CC-BY license.

Chromatin Immunoprecipitation: DDX19B Antibody [NB100-760] -

Chromatin Immunoprecipitation: DDX19B Antibody [NB100-760] - MKL1 contains two Ddx19 interaction sites.(a) Co-IPs of endogenous MKL1 with endogenous Ddx19. NIH 3T3 cytoplasmic extract was immunoprecipitated with anti-Ddx19 antibody & the immunoprecipitates were blotted for indicated antibodies. Beads without antibody (no ab) were used as a negative control. (b) HA-tagged MKL1 constructs were immunoprecipitated from NIH 3T3 lysates & their expression was detected by HA-antibody. Co-precipitated endogenous Ddx19 was detected by western blotting (WB). Empty HA was used as a negative control. Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms6978), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: DDX19B Antibody [NB100-760] -

Western Blot: DDX19B Antibody [NB100-760] - Ddx19 interacts with MKL1 RPEL domain & Ipo beta.(a) Ddx19 recruitment from Hela cell lysate by GST-RPEL & GST-Ipo beta (left). WB, western blotting; (−), without Ran-Q69L; (+), with RanQ69L. Corresponding GST baits are stained with Ponceau to ensure the equal loading of the samples (below). Input sample corresponds to 5% of the Hela cell lysate used in the assay. (b) GST-RPEL was used as a bait for pull down of Ddx19 & Ipo beta from the Hela cell lysate in the presence of increasing amounts of LatB-actin (0.25–10 μM) (left). Arrow indicates the increasing amounts of actin in the corresponding Ponceau staining (below). Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms6978), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: DDX19B Antibody [NB100-760] -

Western Blot: DDX19B Antibody [NB100-760] - The accumulation of viral proteins & RNAs is reduced in DDX19-depleted cells.A549 cells were treated with control (C) or DDX19 (19) siRNAs & infected with WSN (5 pfu/cell). (a) Total extracts were prepared at the indicated times post-infection & analyzed by immunoblots using antibodies directed against the indicated proteins. Results representative of 3 independent experiments are shown. Cropped blots are shown. The corresponding full-length blots are shown in Figure S3. (b–e) The levels of NP or NA mRNAs & vRNAs (b or c & d or e, respectively) were determined at the indicated times post-infection by strand specific RT-qPCR & were normalized to the level of the same RNA species at 3 hpi in cells treated with the control siRNAs. The results are expressed as the mean ± SEM of three independent experiments & the significance was tested with a one-sample t test using GraphPad Prism Software (*p < 0.05; **p < 0.01; ***p < 0.001). Dashed lines were used to indicate that the Y-axes have been segmented. Different scales were used for the mRNA & vRNA graphs. Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/srep33763), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: DDX19B Antibody [NB100-760] -

Western Blot: DDX19B Antibody [NB100-760] - The earliest steps of IAV replication are not affected by DDX19 depletion.A549 cells treated with control (C in a & b, dark grey bars in c & d) or DDX19 (19 in a & b, light grey bars in c & d) siRNAs were infected with WSN (5 pfu/cell). (a) Total extracts from control cells treated with CHX (+CHX) or not (−CHX) were prepared at 6 hpi & analyzed by immunoblots using antibodies directed against the indicated proteins. Cropped blots are shown. The corresponding full-length blots are shown in Figure S4. (b) Cytoplasmic & nuclear fractions were prepared at 4 hpi. Aliquots of the indicated subcellular fractions were analyzed by immunoblots with antibodies directed against MEK1/2 kinase (cytoplasmic marker), TBP (nuclear marker) & NP. Alternatively, total RNAs were extracted & the levels of GAPDH pre-mRNA, a nuclear marker, were determined by real-time RT-PCR. Results are expressed as the mean of two determinations of the crossing point value (Cp). Cropped blots are shown. The corresponding full-length blots are shown in Figure S5. (c,d) Infection was carried out for 6 h in the presence of CHX (100 µg/mL). Total RNAs were isolated from cytoplasmic (solid bars) & nuclear (hatched bars) fractions, & the levels of NP & NA vRNAs were determined by strand specific RT-qPCR. The results are expressed as the mean percentages ± SEM of cytoplasmic & nuclear vRNAs levels determined in three independent experiments (c). Total RNAs were extracted & the levels of NP or NA mRNAs & vRNAs were determined by strand specific RT-qPCR. The results are expressed as the mean ratios of mRNA/vRNA ± SEM determined in three independent experiments (d). Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/srep33763), licensed under a CC-BY license. Not internally tested by Novus Biologicals.