DDX23 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16130

Novus Biologicals, part of Bio-Techne
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NBP2-16130
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DDX23 Antibodies »

Product Summary for DDX23 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human DDX23. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (86%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

96 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DDX23 Antibody

Western Blot: DDX23 Antibody [NBP2-16130]

Western Blot: DDX23 Antibody [NBP2-16130]

Western Blot: DDX23 Antibody [NBP2-16130] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: DDX23 Antibody [NBP2-16130]

Immunocytochemistry/ Immunofluorescence: DDX23 Antibody [NBP2-16130]

Immunocytochemistry/Immunofluorescence: DDX23 Antibody [NBP2-16130] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DDX23 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.
Immunohistochemistry-Paraffin: DDX23 Antibody [NBP2-16130]

Immunohistochemistry-Paraffin: DDX23 Antibody [NBP2-16130]

Immunohistochemistry-Paraffin: DDX23 Antibody [NBP2-16130] - Protein at nucleus on HBL435 xenograft
View 1 more images

Applications for DDX23 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DDX23

This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a component of the U5 snRNP complex; it may facilitate conformational changes in the spliceosome during nuclear pre-mRNA splicing. An alternatively spliced transcript variant has been found for this gene, but its biological validity has not been determined. [provided by RefSeq]

Alternate Names

100 kDa U5 snRNP-specific protein, DEAD (Asp-Glu-Ala-Asp) box polypeptide 23, DEAD box protein 23, EC 3.6.1, EC 3.6.4.13, MGC8416, probable ATP-dependent RNA helicase DDX23, PRP28, PRP28 homolog, PRP28 homolog, yeast, PRP28p homolog, PRPF28, U5 snRNP 100 kD protein, U5-100K, U5-100KD

Gene Symbol

DDX23

UniProt

Q9BUQ8 (Human)

Additional DDX23 Products

Product Documents for DDX23 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DDX23 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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