Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all DDX28 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 500 and the C-terminus (residue 540) of human DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 28 using the numbering given in entry NP_060850.1 (GeneID 55794).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DDX28 Antibody

Western Blot: DDX28 Antibody [NB100-2345]

Western Blot: DDX28 Antibody [NB100-2345]

Western Blot: DDX28 Antibody [NB100-2345] - Detection of Human DDX28 on HeLa whole cell lysate. DDX28 was also immunoprecipitated using rabbit anti-DDX28 antibody NB100-2344.

Applications for DDX28 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:1000-1:5000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DDX28

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene is intronless. It encodes an RNA-dependent ATPase. The encoded protein is localized in the mitochondria and the nucleus, and can be transported between the mitochondria and the nucleus.

Alternate Names

DEAD (Asp-Glu-Ala-Asp) box polypeptide 28, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 28, EC 3.6.1, EC 3.6.4.13, FLJ11282, M, MDDX28probable ATP-dependent RNA helicase DDX28, Mitochondrial DEAD box protein 28, mitochondrial DEAD-box polypeptide 28

Entrez Gene IDs

55794 (Human)

Gene Symbol

DDX28

UniProt

Q9NUL7 (Human)

Additional DDX28 Products

Product Documents for DDX28 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DDX28 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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