DDX43 Antibody (3G12)
Novus Biologicals, part of Bio-Techne | Catalog # H00055510-M07
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 3G12
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
DDX43 (NP_061135.1, 2 a.a. ~ 90 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SHHGGAPKASTWVVASRRSSTVSRAPERRPAEELNRTGPEGYSVGRGGRWRGTSRPPEAVAAGHEELPLCFALKSHFVGAVIGRGGSKI
Specificity
DDX43 - DEAD (Asp-Glu-Ala-Asp) box polypeptide 43 (3G12)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for DDX43 Antibody (3G12)
![Western Blot: DDX43 Antibody (3G12) [H00055510-M07]](https://resources.bio-techne.com/images/products/DDX43-Antibody-3G12-Western-Blot-H00055510-M07-img0008.jpg "Western Blot: DDX43 Antibody (3G12) [H00055510-M07]")
Western Blot: DDX43 Antibody (3G12) [H00055510-M07]
Western Blot: DDX43 Antibody (3G12) [H00055510-M07] - Analysis of DDX43 expression in transfected 293T cell line by DDX43 monoclonal antibody (M07), clone 3G12. Lane 1: DDX43 transfected lysatE (72.9 KDa). Lane 2: Non-transfected lysate.![Immunocytochemistry/ Immunofluorescence: DDX43 Antibody (3G12) [H00055510-M07]](https://resources.bio-techne.com/images/products/DDX43-Antibody-3G12-Immunocytochemistry-Immunofluorescence-H00055510-M07-img0006.jpg "Immunocytochemistry/ Immunofluorescence: DDX43 Antibody (3G12) [H00055510-M07]")
Immunocytochemistry/ Immunofluorescence: DDX43 Antibody (3G12) [H00055510-M07]
Immunocytochemistry/Immunofluorescence: DDX43 Antibody (3G12) [H00055510-M07] - Analysis of monoclonal antibody to DDX43 on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: DDX43 Antibody (3G12) [H00055510-M07] - Detection limit for recombinant GST tagged DDX43 is 0.3 ng/ml as a capture antibody.
Applications for DDX43 Antibody (3G12)
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
10 ug/ml
Western Blot
1:500
Application Notes
Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: DDX43
Alternate Names
Cancer/testis antigen 13, CT13DEAD-box protein 43, DEAD (Asp-Glu-Ala-Asp) box polypeptide 43, DEAD box protein 43, DKFZp434H2114, EC 3.6.1, EC 3.6.4.13, HAGEDEAD box protein HAGE, Helical antigen, probable ATP-dependent RNA helicase DDX43
Entrez Gene IDs
55510 (Human)
Gene Symbol
DDX43
UniProt
Additional DDX43 Products
Product Documents for DDX43 Antibody (3G12)
Product Specific Notices for DDX43 Antibody (3G12)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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