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Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 275 and 325 of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 43 using the numbering given in entry NP_061135.1 (GeneID 55510).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DDX43 Antibody

Immunoprecipitation: DDX43 Antibody [NBP1-28701]

Immunoprecipitation: DDX43 Antibody [NBP1-28701]

Immunoprecipitation: DDX43 Antibody [NBP1-28701] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DDX43 antibody NBP1-28701 used for IP at 10 ug/mg lysate. DDX43 was also immunoprecipitated by rabbit anti-DDX43 antibody NBP1-28719, which recognizes an upstream epitope. For blotting immunoprecipitated DDX43, NBP1-28719 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for DDX43 Antibody

Application
Recommended Usage

Immunoprecipitation

10 ug/mg lysate
Application Notes
It is not recommended for western analysis.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DDX43

DDX43 is a DEAD box ATP-dependent RNA helicase. The DDX43 gene is also known as HAGE. It has been found to be expressed specifically in tumors.

Alternate Names

Cancer/testis antigen 13, CT13DEAD-box protein 43, DEAD (Asp-Glu-Ala-Asp) box polypeptide 43, DEAD box protein 43, DKFZp434H2114, EC 3.6.1, EC 3.6.4.13, HAGEDEAD box protein HAGE, Helical antigen, probable ATP-dependent RNA helicase DDX43

Entrez Gene IDs

55510 (Human)

Gene Symbol

DDX43

Additional DDX43 Products

Product Documents for DDX43 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DDX43 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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