DDX5 Antibody (1K6X10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15371

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15371-100ul
NBP3-15371-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1K6X10

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all DDX5 Antibodies »

Product Summary for DDX5 Antibody (1K6X10)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DDX5 (P17844). MSGYSSDRDRGRDRGFGAPRFGGSRAGPLSGKKFGNPGEKLVKKKWNLDELPKFEKNFYQEHPDLARRTAQEVETYRRSKEITVRGHNCPKPVLNFYEAN

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DDX5 Antibody (1K6X10)

Western Blot: DDX5 Antibody (1K6X10) [NBP3-15371]

Western Blot: DDX5 Antibody (1K6X10) [NBP3-15371]

Western Blot: DDX5 Antibody (1K6X10) [NBP3-15371] - Western blot analysis of extracts of various cell lines, using DDX5 Rabbit mAb (NBP3-15371) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: DDX5 Antibody (1K6X10) [NBP3-15371]

Immunocytochemistry/ Immunofluorescence: DDX5 Antibody (1K6X10) [NBP3-15371]

Immunocytochemistry/Immunofluorescence: DDX5 Antibody (1K6X10) [NBP3-15371] - Immunofluorescence analysis of NIH-3T3 cells using DDX5 Rabbit mAb (NBP3-15371) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: DDX5 Antibody (1K6X10) [NBP3-15371]

Immunohistochemistry-Paraffin: DDX5 Antibody (1K6X10) [NBP3-15371]

Immunohistochemistry-Paraffin: DDX5 Antibody (1K6X10) [NBP3-15371] - Immunohistochemistry of paraffin-embedded mouse spinal cord using DDX5 Rabbit mAb (NBP3-15371) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for DDX5 Antibody (1K6X10)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DDX5

DDX5, also known as Probable ATP-dependent RNA helicase DDX5, is a 69.1 kDa, 613 amino acid protein that regulates pre-mRNA splicing by increasing the inclusion of tau exon 10. Current research with the protein is being conducted for diseases and disorders such as squamous cell carcinoma, breast cancer, fibrosis, prostate cancer, hepatitis, malaria, and myeloma. The protein interacts in translational control and spliceosome pathways with HIST1H4A- HIST1H4E proteins.

Long Name

DEAD Box Protein 5

Alternate Names

DEAD/H, G17P1, HELR, HLR1, HUMP68

Gene Symbol

DDX5

Additional DDX5 Products

Product Documents for DDX5 Antibody (1K6X10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DDX5 Antibody (1K6X10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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