DDX5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB200-351

Novus Biologicals, part of Bio-Techne
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NB200-351
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Hamster

Cited:

Human, Hamster

Predicted:

Primate (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all DDX5 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 575 and the C-terminus (residue 614) of human DEAD (Asp-Glu-Ala-Asp) Polypeptide 5 using the numbering given in entry NP_004387.1 (GeneID 1655).

Reactivity Notes

Hamster reactivity reported in scientific literature (PMID: 28516729). Based on 100% sequence identity, this antibody is predicted to react with Orangutan and Chimpanzee

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DDX5 Antibody

Western Blot: DDX5 Antibody [NB200-351]

Western Blot: DDX5 Antibody [NB200-351]

Western Blot: DDX5 Antibody [NB200-351] - Detection of Human and Mouse DDX5 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DDX5 antibody NB200-351 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.
Immunohistochemistry: DDX5 Antibody [NB200-351]

Immunohistochemistry: DDX5 Antibody [NB200-351]

Immunohistochemistry: DDX5 Antibody [NB200-351] - Detection of human DDX5 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-DDX5 NB200-351 used at a dilution of 1:1000 (0.2ug/ml). Detection: DAB
Western Blot: DDX5 Antibody [NB200-351]

Western Blot: DDX5 Antibody [NB200-351]

Western Blot: DDX5 Antibody [NB200-351] - Detection of DDX5 in whole cell lysates from HeLa (5, 15, 50 ug), 293 (T; 50 ug), and MCF7 (M; 50 ug), and K562 (K; 50 ug) for WB (A) and HeLa (1 mg) for IP (B). Antibodies: NB 200-351 used at 0.04 ug/ml for WB (A & B) and at 3 ug/ml lysate for IP (B). DDX5 was also immonuprecipitated with NB 200-350 used at 3 ug/ml lysate. Detection: ECL 30 seconds.
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Applications for DDX5 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1 ug/ml

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2500-1:10000
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Immunoprecipitation: the antibody may be diluted further than indicated. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Reviewed Applications

Read 1 review rated 5 using NB200-351 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DDX5

DDX belongs to the family of DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, which are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.

Long Name

DEAD Box Protein 5

Alternate Names

DEAD/H, G17P1, HELR, HLR1, HUMP68

Entrez Gene IDs

1655 (Human)

Gene Symbol

DDX5

UniProt

P17844 (Human)

Additional DDX5 Products

Product Documents for DDX5 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for DDX5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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