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DDX55 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47490

Novus Biologicals, part of Bio-Techne
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NBP2-47490
NBP2-47490-25ul

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: KMNEKRKREEGSDIEDEDMEELLNDTRLLKKLKKGKITEEEFEKGLLTTGKRTIKTVDLGISDLEDDC

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DDX55 Antibody

Western Blot: DDX55 Antibody [NBP2-47490]

Western Blot: DDX55 Antibody [NBP2-47490]

Western Blot: DDX55 Antibody [NBP2-47490] - Analysis in human cell line NB4.
Immunocytochemistry/ Immunofluorescence: DDX55 Antibody [NBP2-47490]

Immunocytochemistry/ Immunofluorescence: DDX55 Antibody [NBP2-47490]

Immunocytochemistry/Immunofluorescence: DDX55 Antibody [NBP2-47490] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & nucleoli.
Immunohistochemistry-Paraffin: DDX55 Antibody [NBP2-47490]

Immunohistochemistry-Paraffin: DDX55 Antibody [NBP2-47490]

Immunohistochemistry-Paraffin: DDX55 Antibody [NBP2-47490] - Staining of human testis shows strong nuclear and cytoplasmic positivity in cells in seminiferous ducts.

Applications for DDX55 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DDX55

DDX55 is a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.

Alternate Names

DEAD (Asp-Glu-Ala-Asp) box polypeptide 55, DEAD box protein 55, EC 3.6.1, EC 3.6.4.13, FLJ16577, KIAA1595ATP-dependent RNA helicase DDX55, MGC33209

Gene Symbol

DDX55

Additional DDX55 Products

Product Documents for DDX55 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DDX55 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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