DDX59 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13909

Novus Biologicals, part of Bio-Techne
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NBP2-13909
NBP2-13909-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DDX59 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: VELCGVKIVVVDEADTMLKMGFQQQVLDILENIPNDCQTILVSATIPTSIEQLASQLLHNPVRIITGEKNLPCANVRQIIL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (81%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DDX59 Antibody

Western Blot: DDX59 Antibody [NBP2-13909]

Western Blot: DDX59 Antibody [NBP2-13909]

Western Blot: DDX59 Antibody [NBP2-13909] - Analysis in control (vector only transfected HEK293T lysate) and DDX59 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: DDX59 Antibody [NBP2-13909]

Immunocytochemistry/ Immunofluorescence: DDX59 Antibody [NBP2-13909]

Immunocytochemistry/Immunofluorescence: DDX59 Antibody [NBP2-13909] - Staining of human cell line U-2 OS shows positivity in cytoskeleton (actin filaments).
Immunohistochemistry-Paraffin: DDX59 Antibody [NBP2-13909]

Immunohistochemistry-Paraffin: DDX59 Antibody [NBP2-13909]

Immunohistochemistry-Paraffin: DDX59 Antibody [NBP2-13909] - Staining of human prostate shows strong cytoplasmic and membranous positivity in glandular cells.

Applications for DDX59 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DDX59

Alternate Names

DEAD (Asp-Glu-Ala-Asp) box polypeptide 59, DEAD box protein 59, DKFZp564B1023, EC 3.6.1, EC 3.6.4.13, Zinc finger HIT domain-containing protein 5, ZNHIT5probable ATP-dependent RNA helicase DDX59

Gene Symbol

DDX59

Additional DDX59 Products

Product Documents for DDX59 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DDX59 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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