DDX6 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2818

Novus Biologicals, part of Bio-Techne
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NB100-2818
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all DDX6 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-AEYHSEPVEDEKP corresponding to C-Terminus according to NP_004388.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for DDX6 Antibody

Western Blot: DDX6 Antibody [NB100-2818]

Western Blot: DDX6 Antibody [NB100-2818]

Western Blot: DDX6 Antibody [NB100-2818] - Staining of Daudi (A), Jurkat (B) and HepG2 (C) lysates (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: DDX6 Antibody [NB100-2818]

Immunohistochemistry-Paraffin: DDX6 Antibody [NB100-2818]

Immunohistochemistry-Paraffin: DDX6 Antibody [NB100-2818] - Staining of paraffin embedded Human Thymus. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: DDX6 Antibody [NB100-2818]

Immunohistochemistry-Paraffin: DDX6 Antibody [NB100-2818]

Immunohistochemistry-Paraffin: DDX6 Antibody [NB100-2818] - Staining of paraffin embedded Human Cortex. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for DDX6 Antibody

Application
Recommended Usage

Immunohistochemistry

5 ug/mL

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:128000

Western Blot

0.3 - 1 ug/mL
Application Notes
WB: Approx. 50 kDa band observed in lysates of Daudi, Jurkat and HepG2 (calculated MW of 53.2 kDa band according to NP_004388.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DDX6

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis and cellular growth and division. In the process of mRNA degradation, DDX6 may play a role in mRNA decapping. It forms a complex with DCP1A, DCP2, EDC3 and EDC4/HEDLS

Alternate Names

ATP-dependent RNA helicase p54, DEAD (Asp-Glu-Ala-Asp) box polypeptide 6, DEAD box protein 6, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD), EC 3.6.1, EC 3.6.4.13, FLJ36338, HLR2DEAD box-6, Oncogene RCK, probable ATP-dependent RNA helicase DDX6, RCKP54

Entrez Gene IDs

1656 (Human); 13209 (Mouse)

Gene Symbol

DDX6

UniProt

NP_004388.1 (Human)

Additional DDX6 Products

Product Documents for DDX6 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for DDX6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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