DDX6 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83249

Novus Biologicals, part of Bio-Techne
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NBP1-83249
NBP1-83249-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DDX6 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ENPVIMGLSSQNGQLRGPVKPTGGPGGGGTQTQQQMNQLKNTNTINNGTQQQAQSMTTTIKPGDDWKKTLKLPPKDLRIKTSDVTSTKGNEFEDYCLKRELLMGIFEMGWEKPSPIQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DDX6 Antibody

Western Blot: DDX6 Antibody [NBP1-83249]

Western Blot: DDX6 Antibody [NBP1-83249]

Western Blot: DDX6 Antibody [NBP1-83249] - Analysis using Anti-DDX6 antibody NBP1-83249 (A) shows similar pattern to independent antibody NBP1-83248 (B).
Immunocytochemistry/ Immunofluorescence: DDX6 Antibody [NBP1-83249]

Immunocytochemistry/ Immunofluorescence: DDX6 Antibody [NBP1-83249]

Immunocytochemistry/Immunofluorescence: DDX6 Antibody [NBP1-83249] - Staining of human cell line A-431 shows localization to cytoplasmic bodies.
Immunohistochemistry-Paraffin: DDX6 Antibody [NBP1-83249]

Immunohistochemistry-Paraffin: DDX6 Antibody [NBP1-83249]

Immunohistochemistry-Paraffin: DDX6 Antibody [NBP1-83249] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.
View 4 more images

Applications for DDX6 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DDX6

DDX6 encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing.

Alternate Names

ATP-dependent RNA helicase p54, DEAD (Asp-Glu-Ala-Asp) box polypeptide 6, DEAD box protein 6, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD), EC 3.6.1, EC 3.6.4.13, FLJ36338, HLR2DEAD box-6, Oncogene RCK, probable ATP-dependent RNA helicase DDX6, RCKP54

Gene Symbol

DDX6

Additional DDX6 Products

Product Documents for DDX6 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DDX6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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