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Decorin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84970

Novus Biologicals, part of Bio-Techne
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NBP1-84970

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TNITSIPQGLPPSLTELHLDGNKISRVDAASLKGLNNLAKLGLSFNSISAVDNGSLANTPHLRELHLDNNKLTRVPGGLAEHKYIQVVYLHNNNISVVGSSDFCPPGHNTKKASYSGVSLFSNPVQY

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 24634138).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Decorin Antibody

Immunohistochemistry-Paraffin: Decorin Antibody [NBP1-84970]

Immunohistochemistry-Paraffin: Decorin Antibody [NBP1-84970]

Immunohistochemistry-Paraffin: Decorin Antibody [NBP1-84970] - Staining in human ovary and cerebral cortex tissues using anti-DCN antibody. Corresponding DCN RNA-seq data are presented for the same tissues.
Western Blot: Decorin Antibody [NBP1-84970]

Western Blot: Decorin Antibody [NBP1-84970]

Western Blot: Decorin Antibody [NBP1-84970] - Analysis in control (vector only transfected HEK293T lysate) and DCN over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Western Blot: Decorin Antibody [NBP1-84970]

Western Blot: Decorin Antibody [NBP1-84970]

Western Blot: Decorin Antibody [NBP1-84970] - Analysis in human cell line U-2197.

Applications for Decorin Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
WB reported in scientific literature (PMID: 25979340). For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Decorin

Decorin is encoded by this gene is a small cellular or pericellular matrix proteoglycan that is closely related in structure to biglycan protein. The encoded protein and biglycan are thought to be the result of a gene duplication. This protein is a component of connective tissue, binds to type I collagen fibrils, and plays a role in matrix assembly. It contains one attached glycosaminoglycan chain. This protein is capable of suppressing the growth of various tumor cell lines. There are multiple alternatively spliced transcript variants known for this gene. This gene is a candidate gene for Marfan syndrome.

Alternate Names

DCN, DSPG2, PG-II, PGS2, SLRR1B

Gene Symbol

DCN

Additional Decorin Products

Product Documents for Decorin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Decorin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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