Dectin-2/CLEC6A Antibody (3D1) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27159

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Primate

Applications

CyTOF-ready, Flow (Cell Surface), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Kappa Clone # 3D1

Format

BSA Free

Concentration

1.0 mg/ml

View all Dectin-2/CLEC6A Antibodies »

Product Specifications

Immunogen

A blend of two peptides made to amino acids 44-63 and 90-106 of human Dectin-2.

Reactivity Notes

Bovine, Sheep (83%), Mouse (65%), Rat (52%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3 Kappa

Scientific Data Images for Dectin-2/CLEC6A Antibody (3D1) - BSA Free

Western Blot: Dectin-2/CLEC6A Antibody (3D1)BSA Free [NBP2-27159]

Western Blot: Dectin-2/CLEC6A Antibody (3D1)BSA Free [NBP2-27159]

Western Blot: Dectin-2/CLEC6A Antibody (3D1) [NBP2-27159] - Analysis of Dectin-2 in human heart lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse heart lysate, 4) rat heart lysate and 5) human Ramos cell lysate using Dectin-2 antibody at 2 ug/mL. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunohistochemistry-Paraffin: Dectin-2/CLEC6A Antibody (3D1) - BSA Free [NBP2-27159]

Immunohistochemistry-Paraffin: Dectin-2/CLEC6A Antibody (3D1) - BSA Free [NBP2-27159]

Immunohistochemistry-Paraffin: Dectin-2/CLEC6A Antibody (3D1) [NBP2-27159] - Normal human spleen tissue stained with Dectin-2 antibody (5 ug/mL), peroxidase-conjugate and DAB chromogen. Human tissue TMA was used for this test.
Flow Cytometry: Dectin-2/CLEC6A Antibody (3D1) - BSA Free [NBP2-27159]

Flow Cytometry: Dectin-2/CLEC6A Antibody (3D1) - BSA Free [NBP2-27159]

Flow Cytometry: Dectin-2/CLEC6A Antibody (3D1) [NBP2-27159] - An intracellular stain was performed on THP-1 cells with Dectin-2/CLEC6A (3D1) antibody NBP2-27159AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
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Applications for Dectin-2/CLEC6A Antibody (3D1) - BSA Free

Application
Recommended Usage

Flow Cytometry

0.5 ug/5x10^5 cells

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Western Blot

2 - 5ug/mL

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Dectin-2/CLEC6A

Dectin-2 is a C-type lectin expressed by dendritic cells, macrophages and inflammatory monocytes. It is a type II membrane receptor, with a short cytoplasmic tail, and a C-terminal C-type lectin domain. Dectin-2 is a receptor with specificity for high mannose structures and mediates cytokine production from DC and other antigen presenting cells. Though, Dectin-2 has no obvious signaling motifs attached to CP tail, reports indicate that Dectin-2 employs FcR 7; for internalization and to activate NF-kB pathway for cytokine production. Though it shares only 20-25% of homology with Dectin-1, it functions similarly as pattern recognition receptor (PRR) for fungal infections, to activate both innate (Syk-CARD9 innate signaling pathway) and adaptive immune responses (by activation of DC) to fungal infections. Thus Dectin-2 is a connector molecule of both innate and adaptive immunity functioning at DC level.

Alternate Names

CLEC6A, CLECSF10, Dectin2

Gene Symbol

CLEC6A

UniProt

Q6EIG7

Additional Dectin-2/CLEC6A Products

Product Documents for Dectin-2/CLEC6A Antibody (3D1) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Dectin-2/CLEC6A Antibody (3D1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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