Defensin alpha 5 Antibody (8C8) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80693

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Dot Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 8C8

Format

Azide and BSA Free

Concentration

2.35 mg/ml

View all alpha-Defensin 5 Antibodies »

Product Specifications

Immunogen

Full-length human Defensin alpha 5 [Swiss-Prot# Q01523]. The propeptide is the full-length protein. In the mature form, it is cleaved at residue 63 (alanine).

Localization

Secreted protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

10 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Defensin alpha 5 Antibody (8C8) - Azide and BSA Free

Immunohistochemistry: Defensin alpha 5 Antibody (8C8) - Azide and BSA Free [NBP2-80693]

Immunohistochemistry: Defensin alpha 5 Antibody (8C8) - Azide and BSA Free [NBP2-80693]

Immunohistochemistry: Defensin alpha 5 Antibody (8C8) - Azide and BSA Free [NBP2-80693] - Human skeletal muscle myocytes showing moderate nuclear and faint cytoplasmic staining. Image from the standard format of this antibody.
Flow Cytometry: Defensin alpha 5 Antibody (8C8) - Azide and BSA Free [NBP2-80693]

Flow Cytometry: Defensin alpha 5 Antibody (8C8) - Azide and BSA Free [NBP2-80693]

Flow Cytometry: Defensin alpha 5 Antibody (8C8) - Azide and BSA Free [NBP2-80693] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using Defensin alpha 5 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used. Image from the standard format of this antibody.

Applications for Defensin alpha 5 Antibody (8C8) - Azide and BSA Free

Application
Recommended Usage

Dot Blot

reported in scientific literature

ELISA

reported in scientific literature

Flow Cytometry

1 ug per million cells

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

5-10 ug/ml

Immunohistochemistry-Frozen

reported in scientific literature (PMID 30612153)

Immunohistochemistry-Paraffin

5-10 ug/ml

Western Blot

1:1000
Application Notes
In Western blot a band is observed ~10 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Tris-Glycine, 0.15 M NaCl

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

2.35 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: alpha-Defensin 5

Defensins are a kind of endogenous antimicrobial peptide enriched with cysteine/arginine and are widely distributed in internal organs wherein they are recognized as defenders against invasion by pathogenic organisms. Human alpha-defensins (human alpha-defensins 5, -6, and human neutrophil peptides-1, -2, -3, -4) are a class of small antimicrobial peptides with well characterized antibacterial activity against both Gram +ve as well as Gram-ve bacteria, and certain viruses such as such as HSV, HPV, adenovirus and polyoma virus. Human alpha-defensin 5 (HD5) is a 32 amino acids long protein that shares a common structural feature of alpha-defensin family characterized by three-stranded anti-parallel beta-sheets stabilized by three intra-molecular disulfide bridges. HD5 is primarily expressed in small intestinal Paneth cells or epithelial cells of urino-genital tract, and it is found in cytoplasmic vesicle/secretory vesicle or in secreted form. Peptides HD5(20-94), HD5(23-94) and HD5(29-94) are found within tissues among which HD5(20-94) is the predominant intracellular form. In extracellular milieu, peptides HD5(56-94) and HD5(63-94) are found and among these two peptides, HD5 (63-94) is the most abundant form.

Long Name

Defensin Alpha 5

Alternate Names

DEF5, DEFA5, Defensin alpha 5, HD-5

Gene Symbol

DEFA5

Additional alpha-Defensin 5 Products

Product Documents for Defensin alpha 5 Antibody (8C8) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Defensin alpha 5 Antibody (8C8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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