Defensin alpha 5 Antibody (8C8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11290

Novus Biologicals, part of Bio-Techne
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NBP3-11290
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Key Product Details

Species Reactivity

Human

Applications

Immunoassay, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 8C8

Concentration

0.1 mg/ml

View all alpha-Defensin 5 Antibodies »

Product Specifications

Immunogen

Recombinant ProHD5 (aa 20-94) purified from baculovirus infected Hi5 insect cells

Specificity

The monoclonal antibody 8C8 was raised against recombinant HD5 (aa 20-94) and recognizes the propeptide and the partially processed forms (aa 36-94 and 56-94), but it hardly recognizes mature peptide. No cross reactivity with HD6, lysozyme, or sPLA2 could be detected.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Defensin alpha 5 Antibody (8C8)

Application
Recommended Usage

Immunoassay

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
For Immunohistochemistry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um filtered solution in PBS, 0.1% BSA

Preservative

0.02% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: alpha-Defensin 5

The monoclonal antibody 8C8 recognizes human alpha-defensin 5 (HD5). HD5 is an antimicrobial peptide produced by Paneth cells, specialized epithelial cells of the small intestinal crypt. HD5 is stored in the Paneth cells as propeptide (aa 20-94) and released as mature peptide after trypsin digestion, which is co-secreted from the Paneth cell granules. The mature HD5 (aa 63-94) is protected from further digestion by the proteolysis enzyme. HD5 functions in maintaining the correct microbial balance in the intestine. Transgenic mice that express HD5 are protected from lethal challenge caused by Salmonella typhimurium. Expression levels of HD5 are negatively correlated to intestinal infection. HD5 is a useful marker for intestinal metaplasia of the upper gastrointestinal tract. HD5 expression has also been detected in the upper half of the stratified squamous epithelium of the vagina and ectocervix with increasing intensity toward the lumen. Secreted HD5 is detectable in cervicovaginal lavages, with highest concentrations during the secretory phase of the menstrual cycle. The monoclonal antibody 8C8 was raised against recombinant HD5 (aa 20-94) and recognizes the propeptide and the partially processed forms (aa 36-94 and 56-94), but it hardly recognizes mature peptide. No cross reactivity with HD6, lysozyme, or sPLA2 could be detected.

Long Name

Defensin Alpha 5

Alternate Names

DEF5, DEFA5, Defensin alpha 5, HD-5

Gene Symbol

DEFA5

Additional alpha-Defensin 5 Products

Product Documents for Defensin alpha 5 Antibody (8C8)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Defensin alpha 5 Antibody (8C8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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