DEK Antibody (2C2V8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15257

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15257-100ul
NBP3-15257-20ul
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Key Product Details

Species Reactivity

Human, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2C2V8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all DEK Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 51-200 of human DEK (P35659). SLIVEGKREKKKVERLTMQVSSLQREPFTIAQGKGQKLCEIERIHFFLSKKKTDELRNLHKLLYNRPGTVSSLKKNVGQFSGFPFEKGSVQYKKKEEMLKKFRNAMLKSICEVLDLERSGVNSELVKRILNFLMHPKPSGKPLPKSKKTC

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DEK Antibody (2C2V8)

Western Blot: DEK Antibody (2C2V8) [NBP3-15257]

Western Blot: DEK Antibody (2C2V8) [NBP3-15257]

Western Blot: DEK Antibody (2C2V8) [NBP3-15257] - Western blot analysis of extracts of various cell lines, using DEK antibody (NBP3-15257) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Immunocytochemistry/ Immunofluorescence: DEK Antibody (2C2V8) [NBP3-15257]

Immunocytochemistry/ Immunofluorescence: DEK Antibody (2C2V8) [NBP3-15257]

Immunocytochemistry/Immunofluorescence: DEK Antibody (2C2V8) [NBP3-15257] - Immunofluorescence analysis of C6 cells using DEK Rabbit mAb (NBP3-15257) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: DEK Antibody (2C2V8) [NBP3-15257]

Immunohistochemistry-Paraffin: DEK Antibody (2C2V8) [NBP3-15257]

Immunohistochemistry-Paraffin: DEK Antibody (2C2V8) [NBP3-15257] - Immunohistochemistry of paraffin-embedded human breast cancer using DEK Rabbit mAb (NBP3-15257) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Applications for DEK Antibody (2C2V8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DEK

DEK is a chromatin-binding protein that may serve as an architectural protein at promoter or enhancer sites of particular genes and may be involved in the regulation of transcription and mRNA processing. DEK has been identified as an oncoprotein and an autoantigen.

Alternate Names

D6S231EDEK oncogene (DNA binding), DEK oncogene, protein DEK

Gene Symbol

DEK

Additional DEK Products

Product Documents for DEK Antibody (2C2V8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DEK Antibody (2C2V8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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