DENN/MADD Domain Containing 2D Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-30995

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DENN/MADD Domain Containing 2D Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: PPQDNSGEALKEPERAQEHSLPNFAGGQHFFEYLLVVSLKKKRSEDDYEPIITYQFPKRENLLRGQQEEEERLLKAI

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%), Rat (82%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DENN/MADD Domain Containing 2D Antibody

Western Blot: DENN/MADD Domain Containing 2D Antibody [NBP2-30995]

Western Blot: DENN/MADD Domain Containing 2D Antibody [NBP2-30995]

Western Blot: DENN/MADD Domain Containing 2D Antibody [NBP2-30995] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: MOLT-4
Immunohistochemistry: DENN/MADD Domain Containing 2D Antibody [NBP2-30995]

Immunohistochemistry: DENN/MADD Domain Containing 2D Antibody [NBP2-30995]

Immunohistochemistry: DENN/MADD Domain Containing 2D Antibody [NBP2-30995] - Staining of human urinary bladder shows strong cytoplasmic and membranous positivity in urothelium.
Simple Western: DENN/MADD Domain Containing 2D Antibody [NBP2-30995]

Simple Western: DENN/MADD Domain Containing 2D Antibody [NBP2-30995]

Simple Western: DENN/MADD Domain Containing 2D Antibody [NBP2-30995] - Simple Western lane view shows a specific band for DENN/MADD Domain Containing 2D in 0.2 mg/ml of MOLT-4 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
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Applications for DENN/MADD Domain Containing 2D Antibody

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Simple Western

1:25

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DENN/MADD Domain Containing 2D

Alternate Names

DENN Domain-Containing Protein 2D, DENND2D, RP5-1180E21.2

Gene Symbol

DENND2D

UniProt

Q9H6A0 (Human)

Additional DENN/MADD Domain Containing 2D Products

Product Documents for DENN/MADD Domain Containing 2D Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DENN/MADD Domain Containing 2D Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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