DENND1B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32715

Novus Biologicals, part of Bio-Techne
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NBP1-32715
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DENND1B Antibodies »

Product Summary for DENND1B Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human DENND1B. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DENND1B Antibody

Western Blot: DENND1B Antibody [NBP1-32715]

Western Blot: DENND1B Antibody [NBP1-32715]

Western Blot: DENND1B Antibody [NBP1-32715] - Sample (30 ug of whole cell lysate)A: MOLT4 7. 5% SDS PAGE, antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: DENND1B Antibody [NBP1-32715]

Immunocytochemistry/ Immunofluorescence: DENND1B Antibody [NBP1-32715]

Immunocytochemistry/Immunofluorescence: DENND1B Antibody [NBP1-32715] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Western Blot: DENND1B Antibody [NBP1-32715]

Western Blot: DENND1B Antibody [NBP1-32715]

Western Blot: DENND1B Antibody [NBP1-32715] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.

Applications for DENND1B Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DENND1B

DENND1B is a member of the connecdenn family, which links RAB35 activation with the clathrin machinery. The protein functions as a guanine nucleotide exchange factor for RAB35. DENND1B is present at higher levels in dendritic and natural killer cells but at lower levels in the other myeloid lineage cells.

Alternate Names

C1ORF18, C1orf218, chromosome 1 open reading frame 218, Connecdenn 2, DENN domain-containing protein 1B, DENN/MADD domain containing 1B, DKFZp547O0715, FAM31BMGC103810, FLJ20054, FLJ21179, FLJ33888, member B, Protein FAM31B

Gene Symbol

DENND1B

Additional DENND1B Products

Product Documents for DENND1B Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DENND1B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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