DENND3 [p Thr450] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11946
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr450 conjugated to KLH. (UniProt: D4A2H4)
Modification
p Thr450
Specificity
Specific for endogenous levels of the ~142 kDa DENND3 phosphorylated at Thr450. The immunolabeling is completely eliminated by treatment with gamma-phosphatase.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
142 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for DENND3 [p Thr450] Antibody
![Western Blot: DENND3 [p Thr450] Antibody [NBP3-11946]](https://resources.bio-techne.com/images/products/DENND3-[p-Thr-450]-Antibody-Western-Blot-NBP3-11946-img0001.jpg "Western Blot: DENND3 [p Thr450] Antibody [NBP3-11946]")
Western Blot: DENND3 [p Thr450] Antibody [NBP3-11946]
Western Blot: DENND3 [p Thr 450] Antibody [NBP3-11946] - Western blot of HeLa cell lysate showing specific immunolabeling of the ~142 kDa DENND3 protein phosphorylated at Thr450 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (lambda-Ptase, 800 units/1mg protein for 30 min).Applications for DENND3 [p Thr450] Antibody
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/mL BSA, 50% Glycerol
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: DENND3
Alternate Names
DENN/MADD domain containing 3, KIAA0870DENN domain-containing protein 3
Gene Symbol
DENND3
Additional DENND3 Products
Product Documents for DENND3 [p Thr450] Antibody
Product Specific Notices for DENND3 [p Thr450] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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