DENND5B Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-31971
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse, Avian
Predicted:
Mouse (90%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Cited:
Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: FIWDFIEKVVAYFETTDQILDNEDDVLIQKSSCKTFCHYVNAINTAPRNIGK
Reactivity Notes
Rat (88%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for DENND5B Antibody
Immunocytochemistry/ Immunofluorescence: DENND5B Antibody [NBP2-31971]
Immunocytochemistry/Immunofluorescence: DENND5B Antibody [NBP2-31971] - Staining of human cell line U-2 OS shows localization to nucleoli & microtubules. Antibody staining is shown in green.Immunohistochemistry-Paraffin: DENND5B Antibody [NBP2-31971]
Immunohistochemistry-Paraffin: DENND5B Antibody [NBP2-31971] - Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.Immunohistochemistry-Paraffin: DENND5B Antibody [NBP2-31971]
Immunohistochemistry-Paraffin: DENND5B Antibody [NBP2-31971] - Staining of human testis shows moderate cytoplasmic and membranous positivity in cells in seminiferus ducts.Applications for DENND5B Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Reviewed Applications
Read 1 review rated 4 using NBP2-31971 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: DENND5B
Alternate Names
DENN domain-containing protein 5B, DENN/MADD domain containing 5B, DKFZp686P1174, FLJ41648, FLJ43333, MGC24039, Rab6IP1-like protein
Gene Symbol
DENND5B
UniProt
Additional DENND5B Products
Product Documents for DENND5B Antibody
Product Specific Notices for DENND5B Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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