DEPDC7 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86068
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:TTIPNQDSQLGKENKLYSPARYADALFKSSDIRSASLEDLWENLSLKPANSPHVNISATLSPQVINEVWQEETIGRLLQLVDLPLLDSLLKQQEAVPKIPQPKRQSTMVNSS
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%), Rat (82%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for DEPDC7 Antibody
![Western Blot: DEPDC7 Antibody [NBP1-86068]](https://resources.bio-techne.com/images/products/DEPDC7-Antibody-Western-Blot-NBP1-86068-img0005.jpg "Western Blot: DEPDC7 Antibody [NBP1-86068]")
Western Blot: DEPDC7 Antibody [NBP1-86068]
Western Blot: DEPDC7 Antibody [NBP1-86068] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
![Immunocytochemistry/ Immunofluorescence: DEPDC7 Antibody [NBP1-86068]](https://resources.bio-techne.com/images/products/DEPDC7-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86068-img0004.jpg "Immunocytochemistry/ Immunofluorescence: DEPDC7 Antibody [NBP1-86068]")
Immunocytochemistry/ Immunofluorescence: DEPDC7 Antibody [NBP1-86068]
Immunocytochemistry/Immunofluorescence: DEPDC7 Antibody [NBP1-86068] - Immunofluorescent staining of human cell line A-431 shows positivity in nucleus & nucleoli. Antibody staining shown in green.Applications for DEPDC7 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Western Blot
0.04-0.4 ug/ml
Application Notes
ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: DEPDC7
Alternate Names
DEP domain containing 7, DEP domain-containing protein 7, dJ85M6.4, dJ85M6.4 (novel 58.3 KDA protein), novel 58.3 KDA protein, Protein TR2/D15, TR2
Gene Symbol
DEPDC7
Additional DEPDC7 Products
Product Documents for DEPDC7 Antibody
Product Specific Notices for DEPDC7 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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