Derlin 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-448

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Canine, Chinese Hamster

Cited:

Mouse, Hamster - Cricetulus (Chinese Hamster)

Predicted:

Bovine (100%), Chicken (100%), Rat (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all Derlin 1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to the C-terminal region of human Derlin 1 (between residues 200-251). [UniProt# Q9BUN8]

Reactivity Notes

Predicted to react with Xenopus, bovine, chicken, rat, zebrafish, and mouse based on 100% sequence homology. Mouse reactivity reported in scientific literature (PMID: 25377857)

Localization

Endoplasmic reticulum

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Derlin 1 Antibody

Western Blot: Derlin 1 Antibody [NB100-448]

Western Blot: Derlin 1 Antibody [NB100-448]

Western Blot: Derlin 1 Antibody [NB100-448] - Detection of Derlin protein in canine KRM microsomes.
Immunocytochemistry/ Immunofluorescence: Derlin 1 Antibody [NB100-448]

Immunocytochemistry/ Immunofluorescence: Derlin 1 Antibody [NB100-448]

Immunocytochemistry/Immunofluorescence: Derlin 1 Antibody [NB100-448] - Derlin-1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry: Derlin 1 Antibody [NB100-448]

Immunohistochemistry: Derlin 1 Antibody [NB100-448]

Immunohistochemistry: Derlin 1 Antibody [NB100-448] - Staining of human liver.

Applications for Derlin 1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:2000

Immunohistochemistry

5-10 ug/ml

Immunohistochemistry-Paraffin

5-10 ug/ml

Western Blot

1:1000-1:5000
Application Notes
In Western blot, a band is seen at ~29 kDa. In ICC/IF, ER staining was observed in HeLa cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Derlin 1

Derlin 1 (degradation in endoplasmic reticulum protein 1) belongs to Derlin family and is a functional sub-unit of the ER-associated degradation (ERAD), a ubiquitin/proteasome dependent degradation process for proteins which fails to fold correctly or assemble into oligomeric complexes in ER. Derlin 1 forms a channel which permits the retrotranslocation of misfolded proteins into cytosol from ER wherein they undergo ubiquitination mediated proteasomal degradation. Derlin 1 can generate homo-/hetero-oligomers with DERL2 and DERL3, and interacts with several other proteins such as AMFR, VIMP/SELS, SEL1L, SYVN1 and VCP, as well as with SEL1L-SYVN1 and VCP-VIMP protein complexes. It also interacts with cytomegalovirus US11 protein, NGLY1 and YOD1, RNF103 etc. Expressed ubiquitously, it can get up-regulated in response to ER stress via ERN1-XBP1 pathway of the UPR/unfolded protein response.

Alternate Names

DER-1, DER1Degradation in endoplasmic reticulum protein 1, Der1-like domain family, member 1, Der1-like protein 1, derlin-1, DERtrin-1, FLJ13784, FLJ42092, MGC3067, PRO2577

Gene Symbol

DERL1

Additional Derlin 1 Products

Product Documents for Derlin 1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Derlin 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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