DFF45/ICAD Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-03452

Novus Biologicals, part of Bio-Techne
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NBP3-03452-100ul
NBP3-03452-20ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all DFF45/ICAD Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-331 of human DFF45/ICAD (NP_004392.1). MEVTGDAGVPESGEIRTLKPCLLRRNYSREQHGVAASCLEDLRSKACDILAIDKSLTPVTLVLAEDGTIVDDDDYFLCLPSNTKFVALASNEKWAYNNSDGGTAWISQESFDVDETDSGAGLKWKNVARQLKEDLSSIILLSEEDLQMLVDAPCSDLAQELRQSCATVQRLQHTLQQVLDQREEVRQSKQLLQLYLQALEKEGSLLSKQEESKAAFGEEVDAVDTGISRETSSDVALASHILTALREKQAPELSLSSQDLELVTKEDPKALAVALNWDIKKTETVQEACERELALRLQQTQSLHSLRSISASKASPPGDLQNPKRARQDPT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DFF45/ICAD Antibody - BSA Free

Western Blot: DFF45/ICAD AntibodyBSA Free [NBP3-03452]

Western Blot: DFF45/ICAD AntibodyBSA Free [NBP3-03452]

Western Blot: DFF45/ICAD Antibody [NBP3-03452] - Western blot analysis of extracts of various cell lines, using DFF45/ICAD antibody (NBP3-03452) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
Immunocytochemistry/ Immunofluorescence: DFF45/ICAD Antibody - BSA Free [NBP3-03452]

Immunocytochemistry/ Immunofluorescence: DFF45/ICAD Antibody - BSA Free [NBP3-03452]

Immunocytochemistry/Immunofluorescence: DFF45/ICAD Antibody [NBP3-03452] - Immunofluorescence analysis of HeLa cells using DFF45/ICAD antibody (NBP3-03452). Blue: DAPI for nuclear staining.
Knockout Validated: DFF45/ICAD Antibody - BSA Free [NBP3-03452]

Knockout Validated: DFF45/ICAD Antibody - BSA Free [NBP3-03452]

Knockout Validated: DFF45/ICAD Antibody [NBP3-03452] - Western blot analysis of extracts from normal (control) and DFF45/ICAD knockout (KO) HeLa cells, using DFF45/ICAD antibody (NBP3-03452) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Applications for DFF45/ICAD Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:100

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DFF45/ICAD

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Long Name

DNA Fragmentation Factor, Subunit alpha

Alternate Names

DFF1, DFFA, ICAD

Gene Symbol

DFFA

Additional DFF45/ICAD Products

Product Documents for DFF45/ICAD Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for DFF45/ICAD Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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