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DGAT1 Antibody (SR1829)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-22666

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-22666-100ul
NBP3-22666-50ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1829

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for DGAT1 Antibody (SR1829)

Immunogen

A synthesized peptide derived from human DGAT1 (Uniprot #: O75907)

Specificity

Detects endogenous levels of total DGAT1

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DGAT1 Antibody (SR1829)

Western Blot: DGAT1 Antibody (SR1829) [NBP3-22666] -

Western Blot: DGAT1 Antibody (SR1829) [NBP3-22666] - Western blot analysis using NBP3-22666 in HeLa cell lysate.

Applications for DGAT1 Antibody (SR1829)

Application
Recommended Usage

Flow Cytometry

1:60

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunoprecipitation

1:50

Western Blot

1:1000-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DGAT1

Diglyceride acyltransferase (DGAT) is a membrane-bound Sterol o-acyltransferase subfamily member. DGAT1 is a microsomal enzyme that assists in the synthesis of fatty acids into triglycerides. Specifically, it catalyzes the terminal step in triacylglycerol synthesis, using diacylglycerol (DAG) and fatty acyl CoA as substrates.

DGAT plays a fundamental role in the metabolism of cellular diacylglycerol and is important in higher eukaryotes for physiologic processes involving triacylglycerol metabolism such as intestinal fat absorption, lipoprotein assembly, adipose tissue formation, and lactation. DGAT1 is involved in fat absorption in the intestine and in basal level triglyceride synthesis in adipose tissue, where it is more highly expressed.

Long Name

Diacylglycerol O-Acyltransferase 1

Alternate Names

AGRP1, ARAT, ARGP1, DGAT, DIAR7

Gene Symbol

DGAT1

Additional DGAT1 Products

Product Documents for DGAT1 Antibody (SR1829)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DGAT1 Antibody (SR1829)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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