DGAT1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-57086

Novus Biologicals, part of Bio-Techne
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NB100-57086
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Bovine (100%), Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all DGAT1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QNSMKPFKDMDYS corresponding to internal region according to NP_036211.1.

Epitope

QNSMKPFKDMDYS

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25714675).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

55.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DGAT1 Antibody

Western Blot: DGAT1 Antibody [NB100-57086]

Western Blot: DGAT1 Antibody [NB100-57086]

Western Blot: DGAT1 Antibody [NB100-57086] - Staining of Mouse Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: DGAT1 Antibody [NB100-57086]

Immunocytochemistry/ Immunofluorescence: DGAT1 Antibody [NB100-57086]

Immunocytochemistry/Immunofluorescence: DGAT1 Antibody [NB100-57086] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunohistochemistry-Paraffin: DGAT1 Antibody [NB100-57086]

Immunohistochemistry-Paraffin: DGAT1 Antibody [NB100-57086]

Immunohistochemistry-Paraffin: DGAT1 Antibody [NB100-57086] - Staining of paraffin embedded Human Colon. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Applications for DGAT1 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry-Paraffin

3.75 ug/mL

Peptide ELISA

Detection limit 1:64000

Western Blot

0.01 - 0.1 ug/mL
Application Notes
WB: Approx. 55 kDa band observed in human liver lysates (calculated MW of 55.3 kDa band according to NP_036211.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DGAT1

The enzyme encoded by this gene utilizes diacylglycerol and fatty acyl CoA as substrates in order to catalyze the final stage of triacylglycerol synthesis. It is also involved in cellular as well as physiological metabolic processes.

Long Name

Diacylglycerol O-Acyltransferase 1

Alternate Names

AGRP1, ARAT, ARGP1, DGAT, DIAR7

Entrez Gene IDs

8694 (Human); 13350 (Mouse); 84497 (Rat)

Gene Symbol

DGAT1

UniProt

O75907 (Human)

Additional DGAT1 Products

Product Documents for DGAT1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DGAT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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