DGAT1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92984

Novus Biologicals, part of Bio-Techne
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NBP2-92984-0.02ml
NBP2-92984-0.1ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all DGAT1 Antibodies »

Product Summary for DGAT1 Antibody - BSA Free

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 201-300 of human DGAT1 (NP_036211.2). YELNFPRSPRIRKRFL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DGAT1 Antibody - BSA Free

Western Blot: DGAT1 AntibodyBSA Free [NBP2-92984]

Western Blot: DGAT1 AntibodyBSA Free [NBP2-92984]

Western Blot: DGAT1 Antibody [NBP2-92984] - Analysis of extracts of various cell lines, using DGAT1 antibody (NBP2-92984) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: DGAT1 Antibody - BSA Free [NBP2-92984]

Immunocytochemistry/ Immunofluorescence: DGAT1 Antibody - BSA Free [NBP2-92984]

Immunocytochemistry/Immunofluorescence: DGAT1 Antibody [NBP2-92984] - Analysis of PC-12 cells using DGAT1 Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
Western Blot: DGAT1 AntibodyBSA Free [NBP2-92984]

Western Blot: DGAT1 AntibodyBSA Free [NBP2-92984]

Western Blot: DGAT1 Antibody [NBP2-92984] - Analysis of extracts of Mouse liver, using DGAT1 antibody (NBP2-92984) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
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Applications for DGAT1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DGAT1

Diglyceride acyltransferase (DGAT) is a membrane-bound Sterol o-acyltransferase subfamily member. DGAT1 is a microsomal enzyme that assists in the synthesis of fatty acids into triglycerides. Specifically, it catalyzes the terminal step in triacylglycerol synthesis, using diacylglycerol (DAG) and fatty acyl CoA as substrates.

DGAT plays a fundamental role in the metabolism of cellular diacylglycerol and is important in higher eukaryotes for physiologic processes involving triacylglycerol metabolism such as intestinal fat absorption, lipoprotein assembly, adipose tissue formation, and lactation. DGAT1 is involved in fat absorption in the intestine and in basal level triglyceride synthesis in adipose tissue, where it is more highly expressed.

Long Name

Diacylglycerol O-Acyltransferase 1

Alternate Names

AGRP1, ARAT, ARGP1, DGAT, DIAR7

Gene Symbol

DGAT1

Additional DGAT1 Products

Product Documents for DGAT1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for DGAT1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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