DGK-alpha Antibody (OTI4A11)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-01597

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI4A11

Concentration

1 mg/ml

View all DGK-alpha Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human DGKA(NP_001336) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

82.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DGK-alpha Antibody (OTI4A11)

Western Blot: DGK-alpha Antibody (OTI4A11) [NBP2-01597]

Western Blot: DGK-alpha Antibody (OTI4A11) [NBP2-01597]

Western Blot: DGKA Antibody (4A11) [NBP2-01597] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DGKA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DGKA.
Immunohistochemistry-Paraffin: DGKA Antibody (4A11) [NBP2-01597] Staining of paraffin-embedded Human tonsil using anti-DGKA mouse monoclonal antibody.
Flow Cytometry: DGK-alpha Antibody (OTI4A11) [NBP2-01597]

Flow Cytometry: DGK-alpha Antibody (OTI4A11) [NBP2-01597]

Flow Cytometry: DGKA Antibody (4A11) [NBP2-01597] - Analysis of Jurkat cells, using anti-DGKA antibody, (Red), compared to a nonspecific negative control antibody (Blue).
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Applications for DGK-alpha Antibody (OTI4A11)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DGK-alpha

The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq].

Long Name

Diacylglycerol Kinase, alpha 80kDa

Alternate Names

DAGK1, DGKA, DGKalpha

Entrez Gene IDs

1606 (Human)

Gene Symbol

DGKA

Additional DGK-alpha Products

Product Documents for DGK-alpha Antibody (OTI4A11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DGK-alpha Antibody (OTI4A11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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