DHFRL1 Antibody (OTI1E2) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72459

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Canine, Monkey

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1E2

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all DHFRL1 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 4-185 of human DHFRL1(NP_789785) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

21.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DHFRL1 Antibody (OTI1E2) - Azide and BSA Free

Western Blot: DHFRL1 Antibody (OTI1E2)Azide and BSA Free [NBP2-72459]

Western Blot: DHFRL1 Antibody (OTI1E2)Azide and BSA Free [NBP2-72459]

Western Blot: DHFRL1 Antibody (OTI1E2) - Azide and BSA Free [NBP2-72459] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DHFRL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DHFRL1.
Flow Cytometry: DHFRL1 Antibody (OTI1E2) - Azide and BSA Free [NBP2-72459]

Flow Cytometry: DHFRL1 Antibody (OTI1E2) - Azide and BSA Free [NBP2-72459]

Flow Cytometry: DHFRL1 Antibody (OTI1E2) - Azide and BSA Free [NBP2-72459] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY DHFRL1.
Flow Cytometry: DHFRL1 Antibody (OTI1E2) - Azide and BSA Free [NBP2-72459]

Flow Cytometry: DHFRL1 Antibody (OTI1E2) - Azide and BSA Free [NBP2-72459]

Flow Cytometry: DHFRL1 Antibody (OTI1E2) - Azide and BSA Free [NBP2-72459] - Analysis of extracts (35ug) from 9 different cell lines by using anti-DHFRL1 monoclonal antibody.

Applications for DHFRL1 Antibody (OTI1E2) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DHFRL1

Alternate Names

DHFRP4, dihydrofolate reductase pseudogene 4, dihydrofolate reductase-like 1, dihydrofolate reductase-like protein 1, EC 1.5.1.-, EC 1.5.1.3, FLJ16119

Gene Symbol

DHFR2

Additional DHFRL1 Products

Product Documents for DHFRL1 Antibody (OTI1E2) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DHFRL1 Antibody (OTI1E2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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