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DHRS9 Antibody (3E3)

Novus Biologicals, part of Bio-Techne | Catalog # H00010170-M06

Novus Biologicals, part of Bio-Techne
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H00010170-M06

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3E3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

DHRS9 (AAH58883, 1 a.a. ~ 319 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MLFWVLGLLILCGFLWTRKGKLKIEDITDKYIFITGCDSGFGNLAARTFDKKGFHVIAACLTESGSTALKAETSERLRTVLLDVTDPENVKRTAQWVKNQVGEKGLWGLINNAGVPGVLAPTDWLTLEDYREPIEVNLFGLISVTLNMLPLVKKAQGRVINVSSVGGRLAIVGGGYTPSKYAVEGFNDSLRRDMKAFGVHVSCIEPGLFKTNLADPVKVIEKKLAIWEQLSPDIKQQYGEGYIEKSLDKLKGNKSYVNMDLSPVVECMDHALTSLFPKTHYAAGKDAKIFWIPLSHMPAALQDFLLLKQKAELANPKAV

Specificity

DHRS9 - dehydrogenase/reductase (SDR family) member 9 (3E3)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for DHRS9 Antibody (3E3)

Western Blot: DHRS9 Antibody (3E3) [H00010170-M06]

Western Blot: DHRS9 Antibody (3E3) [H00010170-M06]

Western Blot: DHRS9 Antibody (3E3) [H00010170-M06] - Analysis of DHRS9 expression in transfected 293T cell line by DHRS9 monoclonal antibody (M06), clone 3E3. Lane 1: DHRS9 transfected lysatE (35.2 KDa). Lane 2: Non-transfected lysate.
Immunoprecipitation: DHRS9 Antibody (3E3) [H00010170-M06]

Immunoprecipitation: DHRS9 Antibody (3E3) [H00010170-M06]

Immunoprecipitation: DHRS9 Antibody (3E3) [H00010170-M06] - Analysis of DHRS9 transfected lysate using anti-DHRS9 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with DHRS9 rabbit polyclonal antibody.
Sandwich ELISA: DHRS9 Antibody (3E3) [H00010170-M06] - Detection limit for recombinant GST tagged DHRS9 is 1 ng/ml as a capture antibody.

Applications for DHRS9 Antibody (3E3)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DHRS9

3-alpha-hydroxysteroid dehydrogenase that converts 3-alpha-tetrahydroprogesterone (allopregnanolone) to dihydroxyprogesterone and 3-alpha-androstanediol to dihydroxyprogesterone. May play a role in the biosynthesis of retinoic acid from retinaldehyde, but seems to have low activity with retinoids. Can utilize both NADH and NADPH.

Alternate Names

dehydrogenase/reductase (SDR family) member 9,3alpha-HSD, EC 1.1, EC 1.1.1, EC 1.1.1.105, member 4,3-alpha-HSD, NADP-dependent retinol dehydrogenase/reductase, RDH15, RDH-E2, RDHL3ALPHA-HSD, RDHTBE

Entrez Gene IDs

10170 (Human)

Gene Symbol

DHRS9

Additional DHRS9 Products

Product Documents for DHRS9 Antibody (3E3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DHRS9 Antibody (3E3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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