DHRS9 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97017

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all DHRS9 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human DHRS9 (Accession#: NP_005762.2; Arg18-Val319)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for DHRS9 Antibody

Western Blot: DHRS9 Antibody [NBP2-97017]

Western Blot: DHRS9 Antibody [NBP2-97017]

Western Blot: DHRS9 Antibody [NBP2-97017] - Anti-DHRS9 rabbit polyclonal antibody at 1:500 dilution. Lane A: THP1 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 35 kDa. Observed band size: 35 kDa
Immunoprecipitation: DHRS9 Antibody [NBP2-97017]

Immunoprecipitation: DHRS9 Antibody [NBP2-97017]

Immunoprecipitation: DHRS9 Antibody [NBP2-97017] - DHRS9 was immunoprecipitated using: Lane A: 0.5 mg THP-1 Whole Cell Lysate. 1 ul anti-DHRS9 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-DHRS9 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 35 kDa. Observed band size: 35 kDa

Applications for DHRS9 Antibody

Application
Recommended Usage

ELISA

1:25000-1:50000

Immunoprecipitation

0.2-1 uL/mg of lysate

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DHRS9

3-alpha-hydroxysteroid dehydrogenase that converts 3-alpha-tetrahydroprogesterone (allopregnanolone) to dihydroxyprogesterone and 3-alpha-androstanediol to dihydroxyprogesterone. May play a role in the biosynthesis of retinoic acid from retinaldehyde, but seems to have low activity with retinoids. Can utilize both NADH and NADPH.

Alternate Names

dehydrogenase/reductase (SDR family) member 9,3alpha-HSD, EC 1.1, EC 1.1.1, EC 1.1.1.105, member 4,3-alpha-HSD, NADP-dependent retinol dehydrogenase/reductase, RDH15, RDH-E2, RDHL3ALPHA-HSD, RDHTBE

Gene Symbol

DHRS9

Additional DHRS9 Products

Product Documents for DHRS9 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DHRS9 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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