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DHTKD1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-55215

Novus Biologicals, part of Bio-Techne
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NBP2-55215
NBP2-55215-25ul

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: ALPLFWRGYQTERGVYGYRPRKPESREPQGALERPPVDHGLARLVTVYCEHGHKAAKINPLFTGQALLENVPEIQALVQTLQGPFHTAGL

Reactivity Notes

Rat 80%

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DHTKD1 Antibody

Western Blot: DHTKD1 Antibody [NBP2-55215]

Western Blot: DHTKD1 Antibody [NBP2-55215]

Western Blot: DHTKD1 Antibody [NBP2-55215] - Western blot analysis in human cell line RT-4.
Immunocytochemistry/ Immunofluorescence: DHTKD1 Antibody [NBP2-55215]

Immunocytochemistry/ Immunofluorescence: DHTKD1 Antibody [NBP2-55215]

Immunocytochemistry/Immunofluorescence: DHTKD1 Antibody [NBP2-55215] - Staining of human cell line MCF7 shows localization to mitochondria.
Immunohistochemistry-Paraffin: DHTKD1 Antibody [NBP2-55215]

Immunohistochemistry-Paraffin: DHTKD1 Antibody [NBP2-55215]

Immunohistochemistry-Paraffin: DHTKD1 Antibody [NBP2-55215] - Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Applications for DHTKD1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DHTKD1

The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3)

Alternate Names

dehydrogenase E1 and transketolase domain containing 1, Dehydrogenase E1 and transketolase domain-containing protein 1, DKFZp762M115, EC 1.2.4.2, KIAA1630DKFZP762M115, MGC3090, probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, mitochondrial

Gene Symbol

DHTKD1

Additional DHTKD1 Products

Product Documents for DHTKD1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DHTKD1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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