Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (100%), Orangutan (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all DHX15 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 750 and the C-terminus (residue 795) of human DEAH box polypeptide 15 using the numbering given in Swiss-Prot entry O43143 (GeneID 1665).

Specificity

NB100-586 is specific for human DHX 15 protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DHX15 Antibody

Western Blot: DHX15 Antibody [NB100-586]

Western Blot: DHX15 Antibody [NB100-586]

Western Blot: DHX15 Antibody [NB100-586] - Detection of Human DHX15 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DHX15 antibody NB100-586 used for WB at 0.066 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: DHX15 Antibody [NB100-586]

Western Blot: DHX15 Antibody [NB100-586]

Western Blot: DHX15 Antibody [NB100-586] - Detection of Human DHX15 on HeLa whole cell lysate using NB100-586. Immunoprecipitated DHX15 was blotted using NB100-585 at 0.1 mcg/ml. NB100-585 was also used unsuccessfully for IP.

Applications for DHX15 Antibody

Application
Recommended Usage

Immunoprecipitation

1-5 ug/mg lysate

Western Blot

1:5000-1:15000
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:5,000 - 1:15,000 Immunoprecipitation: 1 to 5 ug/mg lysate Histo/Cytochemistry: not tested *In some cases, the antibody may be diluted further than indicated.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DHX15

The protein encoded by this gene is a putative ATP-dependent RNA helicase implicated in pre-mRNA splicing.

Alternate Names

ATP-dependent RNA helicase #46, DBP1DEAH box protein 15, DDX15putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 15, DEAH (Asp-Glu-Ala-His) box polypeptide 15, EC 3.6.1, EC 3.6.4.13, HRH2DEAD/H box-15, PRP43, PRPF43, PrPp43p, RNA helicase 2

Entrez Gene IDs

1665 (Human)

Gene Symbol

DHX15

UniProt

O43143 (Human)

Additional DHX15 Products

Product Documents for DHX15 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DHX15 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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