DHX36 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84286

Novus Biologicals, part of Bio-Techne
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NBP1-84286
NBP1-84286-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DHX36 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ERREEQIVQLLNSVQAKNDKESEAQISWFAPEDHGYGTEVSTKNTPCSENKLDIQEKKLINQEKKMFRIRNRSYIDRDSEYLLQENEPDGT

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DHX36 Antibody

Western Blot: DHX36 Antibody [NBP1-84286]

Western Blot: DHX36 Antibody [NBP1-84286]

Western Blot: DHX36 Antibody [NBP1-84286] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/ Immunofluorescence: DHX36 Antibody [NBP1-84286]

Immunocytochemistry/ Immunofluorescence: DHX36 Antibody [NBP1-84286]

Immunocytochemistry/Immunofluorescence: DHX36 Antibody [NBP1-84286] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunohistochemistry-Paraffin: DHX36 Antibody [NBP1-84286]

Immunohistochemistry-Paraffin: DHX36 Antibody [NBP1-84286]

Immunohistochemistry-Paraffin: DHX36 Antibody [NBP1-84286] - Staining of human testis shows strong nuclear and cytoplasmic positivity in cells of seminiferus ducts.

Applications for DHX36 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DHX36

DHX36, also known as Probable ATP-dependent RNA helicase DHX36, consists of three isoforms of sizes 114.7 kDa, 113.2 kDa, and 111.5 kDa and is involved in breaking down mRNA with the ARE element in their 3'-UTR strand. Current research links this protein to sex development and spermatogenesis. The protein interacts with RPS9, AGO1, UBC, AGO2, and PHB in the RNA degradation pathway.

Alternate Names

DDX36, DEAH (Asp-Glu-Ala-His) box polypeptide 36, DEAH box protein 36, EC 3.6.1, EC 3.6.4.13, G4 resolvase-1, KIAA1488G4R1, MLEL1RHAUDEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 36, MLE-like protein 1, probable ATP-dependent RNA helicase DHX36, RNA helicase associated with AU-rich element ARE

Gene Symbol

DHX36

Additional DHX36 Products

Product Documents for DHX36 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DHX36 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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