DHX8 Antibody (1E10)

Novus Biologicals, part of Bio-Techne | Catalog # H00001659-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1E10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DHX8 Antibodies »

Product Specifications

Immunogen

DHX8 (NP_004932, 301 a.a. ~ 400 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RREGRVANVADVVSKGQRVKVKVLSFTGTKTSLSMKDVDQETGEDLNPNRRRNLVGETNEETSMRNPDRPTHLSLVSAPEVEDDSLERKRLTRISDPEKW

Specificity

DHX8 (1E10)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for DHX8 Antibody (1E10)

Western Blot: DHX8 Antibody (1E10) [H00001659-M01]

Western Blot: DHX8 Antibody (1E10) [H00001659-M01]

Western Blot: DHX8 Antibody (1E10) [H00001659-M01] - DHX8 monoclonal antibody (M01), clone 1E10 Analysis of DHX8 expression in Hela S3 NE.
Sandwich ELISA: DHX8 Antibody (1E10) [H00001659-M01] - Detection limit for recombinant GST tagged DHX8 is approximately 0.3ng/ml as a capture antibody.

Applications for DHX8 Antibody (1E10)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DHX8

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is highly homologous to yeast Prp22. This protein facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome.

Alternate Names

DDX8ATP-dependent RNA helicase DHX8, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 8 (RNA helicase), DEAH (Asp-Glu-Ala-His) box polypeptide 8, DEAH box protein 8, DEAH-box protein 8, EC 3.6.1, EC 3.6.4.13, HRH1RNA helicase HRH1, PRP22, PRPF22

Entrez Gene IDs

1659 (Human)

Gene Symbol

DHX8

OMIM

600396 (Human)

UniProt

NP_004932 (Human)

Additional DHX8 Products

Product Documents for DHX8 Antibody (1E10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DHX8 Antibody (1E10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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