DHX8 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1714

Novus Biologicals, part of Bio-Techne
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NB100-1714
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all DHX8 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 350 and 400 of human DEAH (Asp-Glu-Ala-His) Box Polypeptide 8 using the numbering given in entry NP_004932.1 (GeneID 1659).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

139 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DHX8 Antibody

Western Blot: DHX8 Antibody [NB100-1714]

Western Blot: DHX8 Antibody [NB100-1714]

Western Blot: DHX8 Antibody [NB100-1714] - Detection of Human DHX8 on HeLa whole cell lystate using NB100-1714. DHX8 was also immunoprecipitated using rabbit anti-DHX8 antibodies NB100-1713 and NB100-1715.

Applications for DHX8 Antibody

Application
Recommended Usage

Immunoprecipitation

1-4 ug/mg of lysate

Western Blot

1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DHX8

DHX8 is a member of the DEAH box family of proteins that possesses several conserved motifs which include the highly conserved DEAH (Asp-Glu-Ala-His) amino acid sequence motif. The major activity of DEAH box proteins is to function as ATP-dependent RNA helicases. RNA helicases play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DHX8 is the human ortholog of the yeast Prp22 protein. DHX8 has been demonstrated to function in the release of RNA from the splicesome and the nuclear export of spliced mRNA. Alternate names for DHX8 include ATP-dependent RNA helicase DHX8, DEAH box protein 8, RNA helicase HRH1, DDX8, HRH1, PRP22, and PRPF22.

Alternate Names

DDX8ATP-dependent RNA helicase DHX8, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 8 (RNA helicase), DEAH (Asp-Glu-Ala-His) box polypeptide 8, DEAH box protein 8, DEAH-box protein 8, EC 3.6.1, EC 3.6.4.13, HRH1RNA helicase HRH1, PRP22, PRPF22

Entrez Gene IDs

1659 (Human)

Gene Symbol

DHX8

UniProt

Q14562 (Human)

Additional DHX8 Products

Product Documents for DHX8 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DHX8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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