DIAPH3 Antibody (4D5)
Novus Biologicals, part of Bio-Techne | Catalog # H00081624-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 4D5
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
DIAPH3 (NP_112194, 632 a.a. ~ 729 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PDILNFVDDLEPLDKASKVSVETLEKNLRQMGRQLQQLEKELETFPPPEDLHDKFVTKMSRFVISAKEQYETLSKLHENMEKLYQSIIGYYAIDVKKV
Specificity
DIAPH3 - diaphanous homolog 3 (Drosophila) (4D5)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for DIAPH3 Antibody (4D5)
![Western Blot: DIAPH3 Antibody (4D5) [H00081624-M01]](https://resources.bio-techne.com/images/products/DIAPH3-Antibody-4D5-Western-Blot-H00081624-M01-img0004.jpg "Western Blot: DIAPH3 Antibody (4D5) [H00081624-M01]")
Western Blot: DIAPH3 Antibody (4D5) [H00081624-M01]
Western Blot: DIAPH3 Antibody (4D5) [H00081624-M01] - Analysis of DIAPH3 expression in transfected 293T cell line by DIAPH3 monoclonal antibody (M01), clone 4D5.Lane 1: DIAPH3 transfected lysate (Predicted MW: 98.1 KDa).Lane 2: Non-transfected lysate.![Immunocytochemistry/ Immunofluorescence: DIAPH3 Antibody (4D5) [H00081624-M01]](https://resources.bio-techne.com/images/products/DIAPH3-Antibody-4D5-Immunocytochemistry-Immunofluorescence-H00081624-M01-img0002.jpg "Immunocytochemistry/ Immunofluorescence: DIAPH3 Antibody (4D5) [H00081624-M01]")
Immunocytochemistry/ Immunofluorescence: DIAPH3 Antibody (4D5) [H00081624-M01]
Immunocytochemistry/Immunofluorescence: DIAPH3 Antibody (4D5) [H00081624-M01] - Analysis of monoclonal antibody to DIAPH3 on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: DIAPH3 Antibody (4D5) [H00081624-M01] - Detection limit for recombinant GST tagged DIAPH3 is 1 ng/ml as a capture antibody.
Applications for DIAPH3 Antibody (4D5)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: DIAPH3
Alternate Names
DIAP3, diaphanous homolog 3 (Drosophila), diaphanous-related formin 3, DKFZp434C0931, DKFZp686A13178, homolog) 3, mDia2, protein diaphanous homolog 3
Entrez Gene IDs
81624 (Human)
Gene Symbol
DIAPH3
UniProt
Additional DIAPH3 Products
Product Documents for DIAPH3 Antibody (4D5)
Product Specific Notices for DIAPH3 Antibody (4D5)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...