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DIAPH3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-30326

Novus Biologicals, part of Bio-Techne
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NBP3-30326

Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Between 1143 and 1193

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DIAPH3 Antibody

DIAPH3 Antibody

Immunoprecipitation:DIAPH3 AntibodyNBP3-30326] -

Immunoprecipitation:DIAPH3 AntibodyNBP3-30326] -Detection of human DIAPH3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DIAPH3 antibody NBP3-30326 (lot NBP3-30326-1) used for IP at 6 µg per reaction. DIAPH3 was also immunoprecipitated by rabbit anti-DIAPH3.. For blotting immunoprecipitated DIAPH3, NBP3-30326 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Applications for DIAPH3 Antibody

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate pH 7 -8.

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DIAPH3

DIAPH3 binds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to themembrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, andtranscriptional activation of the serum r

Alternate Names

DIAP3, diaphanous homolog 3 (Drosophila), diaphanous-related formin 3, DKFZp434C0931, DKFZp686A13178, homolog) 3, mDia2, protein diaphanous homolog 3

Gene Symbol

DIAPH3

Additional DIAPH3 Products

Product Documents for DIAPH3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DIAPH3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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