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Dicer Antibody (GT1176)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13572

Novus Biologicals, part of Bio-Techne
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NBP3-13572

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (99%), Chicken (96%), Mouse (97%), Porcine (99%), Rhesus Macaque (100%), Xenopus (92%), Zebrafish (93%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # GT1176

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for Dicer Antibody (GT1176)

Immunogen

Recombinant protein encompassing a sequence within the center region of human Dicer. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Dicer Antibody (GT1176)

Western Blot: Dicer Antibody (GT1176) [NBP3-13572]

Western Blot: Dicer Antibody (GT1176) [NBP3-13572]

Western Blot: Dicer Antibody (GT1176) [NBP3-13572] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [GT1176] (NBP3-13572) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Dicer Antibody (GT1176) [NBP3-13572]

Immunocytochemistry/ Immunofluorescence: Dicer Antibody (GT1176) [NBP3-13572]

Immunocytochemistry/Immunofluorescence: Dicer Antibody (GT1176) [NBP3-13572] - Dicer antibody [GT1176] detects Dicer protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Dicer stained by Dicer antibody [GT1176] (NBP3-13572) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10um.

Applications for Dicer Antibody (GT1176)

Application
Recommended Usage

Immunohistochemistry

1:10 - 1:50

Immunohistochemistry-Paraffin

1:10 - 1:50

Western Blot

2-5 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Dicer

Dicer encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq]

Alternate Names

DCR1, Dicer, dicer 1, double-stranded RNA-specific endoribonuclease, dicer 1, ribonuclease type III, Dicer1, Dcr-1 homolog (Drosophila), EC 3.1.26, EC 3.1.26.-, EC 3.1.26.3, Helicase MOI, Helicase with RNase motif, helicase-moi, HERNADicer1, Dcr-1 homolog, K12H4.8-LIKE, KIAA0928endoribonuclease Dicer

Gene Symbol

DICER1

Additional Dicer Products

Product Documents for Dicer Antibody (GT1176)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Dicer Antibody (GT1176)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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