Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5B2

Format

BSA Free

Concentration

1 mg/ml

View all Dihydrofolate Reductase/DHFR Antibodies »

Product Specifications

Immunogen

Recombinant human Dihydrofolate Reductase/DHFR (1-187aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Dihydrofolate Reductase/DHFR Antibody (5B2) - BSA Free

Western Blot: Dihydrofolate Reductase/DHFR Antibody (5B2)BSA Free [NBP2-59464]

Western Blot: Dihydrofolate Reductase/DHFR Antibody (5B2)BSA Free [NBP2-59464]

Western Blot: Dihydrofolate Reductase/DHFR Antibody (5B2) [NBP2-59464] - The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DHFR antibody (1:1000). Proteins werevisualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : Jurkat cell lysate Lane 3 : 293T cell lysate
Immunocytochemistry/ Immunofluorescence: Dihydrofolate Reductase/DHFR Antibody (5B2) - BSA Free [NBP2-59464]

Immunocytochemistry/ Immunofluorescence: Dihydrofolate Reductase/DHFR Antibody (5B2) - BSA Free [NBP2-59464]

Immunocytochemistry/Immunofluorescence: Dihydrofolate Reductase/DHFR Antibody (5B2) [NBP2-59464] - Analysis of DHFR in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human DHFR antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Applications for Dihydrofolate Reductase/DHFR Antibody (5B2) - BSA Free

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Dihydrofolate Reductase/DHFR

Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia.

Alternate Names

DHFR, DHFRP1, DYR

Gene Symbol

DHFR

Additional Dihydrofolate Reductase/DHFR Products

Product Documents for Dihydrofolate Reductase/DHFR Antibody (5B2) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Dihydrofolate Reductase/DHFR Antibody (5B2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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