Dihydrolipoamide Dehydrogenase/DLD Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19361

Novus Biologicals, part of Bio-Techne
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NBP2-19361
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Rat

Cited:

Human

Predicted:

Bovine (95%), Canine (97%), Mouse (93%), Porcine (96%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Dihydrolipoamide Dehydrogenase/DLD Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Dihydrolipoamide Dehydrogenase/DLD. The exact sequence is proprietary.

Reactivity Notes

Chicken (88%), Xenopus laevis (80%).

Localization

Mitochondrion matrix

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Dihydrolipoamide Dehydrogenase/DLD Antibody

Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP2-19361]

Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP2-19361]

Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP2-19361] - Wild-type (WT) and DLD knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DLD antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP2-19361]

Immunohistochemistry-Paraffin: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP2-19361]

Immunohistochemistry-Paraffin: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP2-19361] - Analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.
Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP2-19361]

Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP2-19361]

Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP2-19361] - Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE gel, diluted at 1:1000.
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Applications for Dihydrolipoamide Dehydrogenase/DLD Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Dihydrolipoamide Dehydrogenase/DLD

This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. [provided by RefSeq]

Alternate Names

Diaphorase, DLD, DLDD, DLDH, GCSL, LAD, Lipoamide Dehydrogenase, PHE3

Gene Symbol

DLD

UniProt

P09622

Additional Dihydrolipoamide Dehydrogenase/DLD Products

Product Documents for Dihydrolipoamide Dehydrogenase/DLD Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Dihydrolipoamide Dehydrogenase/DLD Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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