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DIMT1L Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-30659

Novus Biologicals, part of Bio-Techne
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NBP2-30659
NBP2-30659-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: GGLMFNTGIGQHILKNPLIINSIIDKAALRPTDVVLEVGPGTGNMTVKLLEKAKKVVACELDPRLVAELHKRVQGTPVASKLQVLVGDVLKTDLPFFDTCV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DIMT1L Antibody

Western Blot: DIMT1L Antibody [NBP2-30659]

Western Blot: DIMT1L Antibody [NBP2-30659]

Western Blot: DIMT1L Antibody [NBP2-30659] - Analysis in human cell line RPMI-8226.
Immunocytochemistry/ Immunofluorescence: DIMT1L Antibody [NBP2-30659]

Immunocytochemistry/ Immunofluorescence: DIMT1L Antibody [NBP2-30659]

Immunocytochemistry/Immunofluorescence: DIMT1L Antibody [NBP2-30659] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus, nucleoli & cytosol.
Immunohistochemistry-Paraffin: DIMT1L Antibody [NBP2-30659]

Immunohistochemistry-Paraffin: DIMT1L Antibody [NBP2-30659]

Immunohistochemistry-Paraffin: DIMT1L Antibody [NBP2-30659] - Staining of human squamous epithelia shows weak positivity in nucleoli in squamous epithelial cells.

Applications for DIMT1L Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DIMT1L

Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle

Alternate Names

18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase, DIM1, DIM1 dimethyladenosine transferase 1-like, DIM1 dimethyladenosine transferase 1-like (S. cerevisiae), DIMT1dimethyladenosine transferase, EC 2.1.1, EC 2.1.1.14, EC 2.1.1.183,18S rRNA dimethylase, HSA9761, HUSSY5, N'-adenosyl(rRNA) dimethyltransferase, probable dimethyladenosine transferase, S-adenosylmethionine-6-N'

Gene Symbol

DIMT1

UniProt

Additional DIMT1L Products

Product Documents for DIMT1L Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DIMT1L Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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