DIO3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB110-96414

Novus Biologicals, part of Bio-Techne
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NB110-96414
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Hamster

Cited:

Human, Mouse, Rat, Hamster

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all DIO3 Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an internal portion of rat DIO3 (within residues 1-100). [Swiss-Prot# P49897]

Reactivity Notes

Human reactivity was confirmed in PMID 22723689.

Localization

Cell membrane; Single-pass type II membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DIO3 Antibody

Western Blot: DIO3 Antibody [NB110-96414]

Western Blot: DIO3 Antibody [NB110-96414]

Western Blot: DIO3 Antibody [NB110-96414] - Detection of Dio3 in rat placenta
Immunohistochemistry-Paraffin: DIO3 Antibody [NB110-96414]

Immunohistochemistry-Paraffin: DIO3 Antibody [NB110-96414]

Immunohistochemistry-Paraffin: DIO3 Antibody [NB110-96414] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human placenta using 1:50 dilution of DIO3 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining in decidual cells was observed. Staining was performed by Histowiz.

Applications for DIO3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500. Use reported in scientific literature (PMID 21828183)

Western Blot

0.5 ug/ml
Application Notes
In Western blot, a band is seen ~31 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DIO3

DIO3 (type III iodothyronine deiodinase), an iodothyronine deiodinase family member, is expressed in placenta and several fetal tissues in the plasma/endosomal membranes as single-pass type II membrane protein, and it catalyzes the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into RT3 (3,3',5'-triiodothyronine) and of T3 (3,5,3'-triiodothyronine) into T2 (3,3'-diiodothyronine), both RT3 and T2 being inactive metabolites thus preventing premature exposure of developing fetus to adult levels of thyroid hormones. DIO3 controls circulating thyroid hormone levels throughout gestation by controlling thyroid hormone inactivation during embryological development. DIO3 is homodimeric and may undergo minor heretodimerization with DIO1 or DIO2, homo- or heterodimer state being critical for its catalytic activity. Its activity has been identified in only a limited number of postnatal tissues, i.e. brain, skin, and pregnant uterus, whereas it is abundantly present in several fetal tissues. Furthermore, DIO3 re-expression has been shown in certain pathologies that include cancer, cardiac hypertrophy, myocardial infarction, chronic inflammation, and critical illness.

Alternate Names

5DIII, D3, deiodinase, iodothyronine, type III, DIOIII, EC 1.97.1, EC 1.97.1.11, ITDI3, placental type, thyroxine deiodinase type III (selenoprotein), Type 3 DI, type 3 iodothyronine selenodeiodinase, type III iodothyronine deiodinase, type-III 5' deiodinase, Type-III 5'-deiodinase

Gene Symbol

DIO3

Additional DIO3 Products

Product Documents for DIO3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DIO3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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