DISC1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB110-40775

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.95 mg/ml

View all DISC1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal region (within residues 700-800) of the mouse DISC1 protein. [Swiss-Prot# Q811T9]

Localization

DISC-1 localizes to the nucleus, whereas mutant DISC-1 localization occurs mainly in the cytoplasm. It co-localizes with the following organelle structure antibodies: PD1, calnexin, GIANTIN, 58K, Rab4, LAMP-1, RAP1, KDEL, and catalase.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DISC1 Antibody

Immunocytochemistry/ Immunofluorescence: DISC1 Antibody [NB110-40775]

Immunocytochemistry/ Immunofluorescence: DISC1 Antibody [NB110-40775]

Immunocytochemistry/Immunofluorescence: DISC1 Antibody [NB110-40775] - DISC1 antibody was tested in PC-12 cells at 1:100 with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 549 (red).
Immunohistochemistry: DISC1 Antibody [NB110-40775]

Immunohistochemistry: DISC1 Antibody [NB110-40775]

Immunohistochemistry: DISC1 Antibody [NB110-40775] - Staining of DISC1 in mouse brain neurons, glial cells, and white matter. 40X

Applications for DISC1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

2.5-5.0 ug/ml

Immunohistochemistry-Frozen

reported in scientific literature (PMID 24560582)

Immunohistochemistry-Paraffin

2.5-5ug/ml
Application Notes
The theoretical molecular weight of DISC1 is ~90 kDa. Preliminary Western Blot studies have been performed with this antibody, where several non-specific bands are seen. Due to alternative splicing, DISC1 may also run at ~75 and 100 kDa. In ICC/IF, cytoplasmic staining was observed in PC12 cells.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Glycine and 0.15M NaCl

Preservative

0.05% Sodium Azide

Concentration

0.95 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DISC1

DISC1 (Disrupted in Schizophrenia 1) is a well known risk genes for psychiatric disorders including schizophrenia, major depression, bipolar disorder, and autism. DISC1 protein localize to cytoskeleton-rich regions in cells, including the centrosome, base of primary cilia, axon and dendritic shafts and spines. For serving its multiple functions, DISC1 interacts with CCDC88A, GSK3B, PCNT, tubulin alpha, ACTN2, ANKHD1, ATF4, ATF5, CEP63, EIF3S3, MAP1A, NDEL1, PAFAH1B1, RANBP9, SPTBN4, SYNE1, TRAF3IP1, CHCHD6 and NDEL1. DISC1 interaction with microtubules TRAF3IP1. In neurogenesis regulation, DISC1 is essential for neural progenitor proliferation in the ventrical/subventrical zone during embryonic brain development as well as in adult dentate gyrus of hippocampus. DISC1 modulates GSK3Beta activity and CTNNB1 abundance for acting as positive regulator of WNT-mediated neural progenitor proliferation. DISC1 can modulate AKT-mTOR signaling for controlling the neuron positioning, dendritic development and synapse formation which ultimately regulate newborn neurons integration during adult neurogenesis and can regulate the migration of early-born granule cell precursors toward the dentate gyrus during the hippocampal development. Moreover, DISC1 is implicated in microtubule network formation, regulation of functional biology of centrosome as well as primary cilia, neuronal migration and microtubule mediated transport.

Long Name

Disrupted in Schizophrenia 1

Alternate Names

C1orf136, SCZD9

Gene Symbol

DISC1

Additional DISC1 Products

Product Documents for DISC1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for DISC1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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