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Dishevelled-2 Antibody (OTI2D11) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70611

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI2D11

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Summary for Dishevelled-2 Antibody (OTI2D11) - Azide and BSA Free

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-320 of human DVL2(NP_004413) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

78.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Dishevelled-2 Antibody (OTI2D11) - Azide and BSA Free

Immunohistochemistry: Dishevelled-2 Antibody (OTI2D11) - Azide and BSA Free [NBP2-70611]

Immunohistochemistry: Dishevelled-2 Antibody (OTI2D11) - Azide and BSA Free [NBP2-70611]

Immunohistochemistry: Dishevelled-2 Antibody (OTI2D11) - Azide and BSA Free [NBP2-70611] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Dishevelled-2 Antibody (OTI2D11) - Azide and BSA Free [NBP2-70611]

Immunohistochemistry: Dishevelled-2 Antibody (OTI2D11) - Azide and BSA Free [NBP2-70611]

Immunohistochemistry: Dishevelled-2 Antibody (OTI2D11) - Azide and BSA Free [NBP2-70611] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120 degrees C for 3min)
Immunohistochemistry: Dishevelled-2 Antibody (OTI2D11) - Azide and BSA Free [NBP2-70611]

Immunohistochemistry: Dishevelled-2 Antibody (OTI2D11) - Azide and BSA Free [NBP2-70611]

Immunohistochemistry: Dishevelled-2 Antibody (OTI2D11) - Azide and BSA Free [NBP2-70611] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Applications for Dishevelled-2 Antibody (OTI2D11) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Dishevelled-2

The Dishevelled 2 gene encodes a member of the dishevelled (dsh) protein family. The vertebrate dsh proteins have approximately 40% amino acid sequence similarity with Drosophila dsh. This gene encodes a 90-kD protein that undergoes posttranslational phosphorylation t

Alternate Names

Dishevelled2, DVL2

Gene Symbol

DVL2

Additional Dishevelled-2 Products

Product Documents for Dishevelled-2 Antibody (OTI2D11) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Dishevelled-2 Antibody (OTI2D11) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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